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Infectious Diseases in Obstetrics and Gynecology
Volume 2011, Article ID 851485, 9 pages
http://dx.doi.org/10.1155/2011/851485
Research Article

Vaginal Microbiota of Healthy Pregnant Mexican Women is Constituted by Four Lactobacillus Species and Several Vaginosis-Associated Bacteria

1Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Plan de Ayala s/n esq. Prol. Manuel Carpio, Colonia Santo Tomás, C.P. 11340 México, DF, Mexico
2Departamento de Salud, Universidad Iberoamericana, Ciudad de México, Prol. Paseo de la Reforma 880, Colonia Lomas de Santa Fe, C.P. 01219 México, DF, Mexico
3Dirección de Enseñanza, Instituto Nacional de Perinatología “Isidro Espinosa de los Reyes” Montes Urales 800, Colonia Lomas Virreyes, C.P. 11000 México, DF, Mexico
4Departamento de Infectologia e Inmunología Perinatal, Instituto Nacional de Perinatología “Isidro Espinosa de los Reyes” Montes Urales 800, Colonia Lomas Virreyes, C.P. 11000 México, DF, Mexico
5Laboratorio de la Dirección de Investigación, Instituto Nacional de Perinatología “Isidro Espinosa de los Reyes” Montes Urales 800, Colonia Lomas Virreyes, C.P. 11000 México, DF, Mexico

Received 15 September 2010; Revised 31 December 2010; Accepted 31 May 2011

Academic Editor: Harold Wiesenfeld

Copyright © 2011 César Hernández-Rodríguez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To identify the microbiota communities in the vaginal tracts of healthy Mexican women across the pregnancy. Methods. Vaginal swabs were obtained during the prenatal visit of women from all trimesters ( 𝑛 = 6 4 ) of healthy pregnant women of Mexico City. DNA was isolated from each sample, and PCR-DGGE and sequencing of 16S rRNA gene fragments were used to identify the bacterial communities. Results. 21 different microorganisms were identified in the vaginal samples. Lactobacillus genus was present in 98% of women studied. Four lactobacilli species were identified in vaginal samples. L. acidophilus was the predominant (78%) followed by L. iners (54%), L. gasseri (20%), and L. delbrueckii (6%). 17 different microorganisms related to bacterial vaginosis conditions were identified. Ureaplasma urealyticum was the predominant (21%) followed by BVAB1 (17%) and Gemella bergeriae (7.8%). Conclusions. Lactobacillus genus predominates in the vaginal samples of Mexican pregnant women associated with different microorganisms related to bacterial vaginosis conditions.