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Infectious Diseases in Obstetrics and Gynecology
Volume 2012 (2012), Article ID 727248, 4 pages
http://dx.doi.org/10.1155/2012/727248
Research Article

In Vitro Anti-Candida Activity of Lidocaine and Nitroglycerin: Alone and Combined

1Health Sciences Research Center (CICS), Faculty of Health Sciences, University of Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal
2Clinical Pathology Laboratory, Hospital Center Cova da Beira, Covilhã, Portugal
3Women and Child Health Department, Hospital Center Cova da Beira, Covilhã, Portugal

Received 9 February 2012; Accepted 19 March 2012

Academic Editor: Secondo Guaschino

Copyright © 2012 Ana Palmeira-de-Oliveira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this work was to study the anti-Candida activity of lidocaine and nitroglycerin alone and in combination. Ten Candida strains were included, corresponding to 1 collection type strain (ATCC 10231) and 9 clinical isolates: 4 C. albicans, 2 C. glabrata, 1 C. tropicalis, 1 C. krusei, and 1 C. parapsilosis. The CLSI reference M27-A3 micromethod was used to determine the anti-Candida activity of the drugs alone; minimal inhibitory and lethal concentrations were determined. The classic checkboard technique was used to determine the activity of combined drugs. Lidocaine fungicidal effect was dosedependent. Nitroglycerin exhibited a higher effect. The drugs combination resulted in a reduction of the inhibitory concentration, corresponding to an additive effect. In conclusion, both drugs exhibited an interesting anti-Candida activity. The combination of lidocaine with nitroglycerin was shown to have an additive effect against Candida spp., predicting the interest to include, in the future, these drugs in a new delivery system for the treatment of mucocutaneous candidosis.