CD8+CXCR5+ T Cells Regulate Pathology in the Genital Tract
Figure 1
CD8+CXCR5+ cells express the phenotype of Tregs. MLNs were isolated from C57BL/6 WT mice 7 days after genital infection with C. muridarum and stained with CD3, CD8, CXCR5, and various markers of cytotoxic and regulatory T cells. (a) The dotplot shows the percentage of CD8 and CXCR5 on CD3+ cells. (b) Histogram of CD3+CD8+CXCR5+ cells showing the individual expression of perforin (gray line), granzyme B (dash line), and CD44 (black line). Irrelevant control antibody stained cells appear as a shaded histogram. (c) Dotplot showing the percent of CD3+CD8+CXCR5+ cells expressing of FoxP3 and CD25 or FoxP3 and GITR following stimulation of spleen cells from mice during genital infection with C. muridarum. (d) Dotplot showing the percent of CD3+CD8+CXCR5+ cells expressing of FoxP3 and CD25 or FoxP3 and GITR following stimulation of spleen cells from naive mice.
