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Infectious Diseases in Obstetrics and Gynecology
Volume 2017, Article ID 6725168, 5 pages
https://doi.org/10.1155/2017/6725168
Research Article

Effect of Genital Sampling Site on the Detection and Quantification of Ureaplasma Species with Quantitative Polymerase Chain Reaction during Pregnancy

1Department of Obstetrics and Prenatal Medicine, Universitair Ziekenhuis Brussel, 101 Laarbeeklaan, Jette, 1090 Brussels, Belgium
2Department of Microbiology, UZ Brussel, 101 Laarbeeklaan, Jette, 1090 Brussels, Belgium
3Department of Biostatistics and Medical Informatics, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, 101 Laarbeeklaan, Jette, 1090 Brussels, Belgium

Correspondence should be addressed to Gilles Faron; eb.lessurbzu@noraf.sellig

Received 19 September 2016; Accepted 5 January 2017; Published 5 February 2017

Academic Editor: Diane M. Harper

Copyright © 2017 Gilles Faron et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. This study aimed to compare the qualitative and quantitative reproducibility of quantitative PCR (qPCR) for Ureaplasma species (Ureaplasma spp.) throughout pregnancy and according to the genital sampling site. Study Design. Between 5 and 14 weeks of gestation (T1), vaginal, fornix, and two cervical samples were taken. Sampling was repeated during the 2nd (T2) and 3rd (T3) trimester in randomly selected T1 positive and negative women. Qualitative and quantitative reproducibility were evaluated using, respectively, Cohen’s kappa (κ) and interclass correlation coefficients (ICC) and repeated measures ANOVA on the log-transformed mean number of DNA copies for each sampling site. Results. During T1, 51/127 women were positive for U. parvum and 8 for U. urealyticum (4 patients for both). Sampling was repeated for 44/55 women at T2 and/or T3; 43 (97.7%) remained positive at the three timepoints. κ ranged between 0.83 and 0.95 and the ICC for cervical samples was 0.86. Conclusions. Colonization by Ureaplasma spp. seems to be very constant during pregnancy and vaginal samples have the highest detection rate.