Molecular Antimicrobial Resistance of<i> Neisseria gonorrhoeae</i> in a Moroccan Area

Karim, Safae; Bouchikhi, Chahrazed; Banani, Abdelaziz; El Fatemi, Hinde; Souho, Tiatou; Erraghay, Sanaa; Bennani, Bahia

doi:https://doi.org/10.1155/2018/7263849

Infectious Diseases in Obstetrics and Gynecology

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Abstract Introduction Materials and Methods Results Discussion Conclusion Data Availability Ethical Approval Conflicts of Interest Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2018 | Article ID 7263849 | https://doi.org/10.1155/2018/7263849

Molecular Antimicrobial Resistance of Neisseria gonorrhoeae in a Moroccan Area

Safae Karim,^1,2Chahrazed Bouchikhi,^3,4Abdelaziz Banani,³Hinde El Fatemi,⁵Tiatou Souho,¹Sanaa Erraghay,³and Bahia Bennani^1,4

Academic Editor: Louise Hafner

Received26 Sept 2018

Revised24 Oct 2018

Accepted11 Nov 2018

Published21 Nov 2018

Abstract

Objectives. To identify the prevalence and the types of Neisseria gonorrhoeae (NG) resistance plasmids-mediated penicillin (PPNG) and tetracycline (TRNG), the ciprofloxacin resistance (CRNG), and related risk factors of each types of resistance. Methods. The beta-lactamase-producing plasmid types (Africa, Asia, and Toronto), tetM tetracycline resistance plasmid types (America and Dutch), and the determination of the Ser-91 mutation of GyrA were detected by specifics PCRs on 149 diagnosed NG positives samples followed by Hinf1 digestion for tetM and gyrA mutation. Results. 135 (90.1%) samples showed a profile of molecular resistance to at least one antibiotic with predominance of ciprofloxacin resistance. In fact, 36 (24.2%) and 69 (46.3%) cases harbored PPNG and TRNG, respectively, and 116 (77.9%) cases showed the mutation Ser-91 of GyrA (CRNG). From a total of 36 PPNG isolates, the Toronto, Asian, and Toronto/Asian types were detected in 13 (36.1%), 10 (27.8%), and 13 (36.1%) cases, respectively, whereas the African type was not detected. In addition, the American type of TRNG was detected in 92.8% (64/69) of cases, while the Dutch type was detected in 7.2% (5/69) of cases. The association of demographics and clinical variables with NG resistance to ciprofloxacin, penicillin, and tetracycline was studied and the risk factors have been determined. Conclusion. Resistance to penicillin, tetracycline, and ciprofloxacin among NG samples positives remained at high levels in Morocco as determined by molecular profile. So, the use of molecular tools for NG antimicrobial resistance detection can help in the management and spread limitation of this infection.

1. Introduction

Neisseria gonorrhoeae (NG) is the second most bacterial sexually transmitted infection (STI) in the world [1]. It can infect the male and female genital tract and cause severe complications such as pelvic inflammatory diseases with possible sequelae in women, infertility in both genders, and neonatal conjunctivitis. Untreated gonorrhea can lead to inflammation of the mucous membranes and facilitates HIV transmission [2].

In 2012, WHO estimated that 78.3 million new gonorrhea cases occurred among women and men aged between 15 and 49 years [1]. Currently, there is no effective vaccine against gonococcal infections. However, infections and NG have developed antimicrobial resistance that has been used previously for its treatment, including to sulfonamides, penicillins, narrow-spectrum cephalosporins, tetracyclines, macrolides, and fluoroquinolones [3]. The plasmid-mediated penicillin-resistant NG (PPNG) has been largely described with high rates that may exceed 80% in some Asian countries [4]. This resistance is mostly associated with three types of β- lactamase producing plasmids: Asian, African, and Toronto types [5]. Also, plasmid-mediated tetracycline-resistant (TRNG) has been described with high rates that may exceed 70% in South Africa [6]. In fact, this resistance by TetM is carried by two conjugative plasmids (American and Dutch) of similar sizes [7], but with different tetM sequences and hence different profiles of endonuclease restriction fragments. However, the last years (2004-2012) were marked by high frequency of ciprofloxacin resistance that reached 99% [8]. This resistance is mainly related to gyrA and parC mutations with predominance of the mutation Ser-91 of GyrA [9]. Due to the emerging resistance of NG, Centers for Disease Control and Prevention have recommended several changes to the treatment guidelines (CDC). In 2012, the single-dose oral cephalosporin has been replaced by dual therapy: a single injection of ceftriaxone (250 mg) and single-dose azithromycin (1 g) administered orally.

In Morocco, a 2009 study conducted in men with urethral discharge showed high rates of penicillin, tetracycline, and ciprofloxacin NG resistance (56.2%, 92.6%, and 86.8%, respectively) [10]. On the basis of these results, the treatment of gonococcal infections was changed and the Ministry of Health replaced ciprofloxacin by single-dose ceftriaxone 250 mg [10]. However, this study was conducted on small sampling (76 cases) that is not representative of all Moroccan regions and in our knowledge, no study has been conducted to determine the mechanisms of antimicrobial resistance of NG circulating in Morocco.

The aims of this study were to identify the prevalence and the types of NG plasmids-mediated penicillinase-producing (PPNG) and Tetracycline-producing (TRNG) and the ciprofloxacin Ser-91 status in gyrA (CRNG) and to identify the associated risk factors.

2. Materials and Methods

2.1. Sampling

From 2013 to 2015, a prospective study was conducted among women consulting the Gynecology Department and the Anatomy-Pathology Laboratory of the Hassan II University Hospital of Fez to determine the prevalence and characterize bacterial STI notably NG. A total of 1053 patients aged 18 years and above, HIV negatives, not virgins, who not menses at the day of sampling and who accepted to provide their written consent were included in this previous study. From all collected cervical samples, 149 (14.1%) were tested positive for NG by PCR using specific primers [12] and are used in the present study to determine the molecular antimicrobial resistance of NG. The deidentified demographics and clinical data of participants are available. Only 31.5% (47/149) of those samples NG positives were obtained in symptomatic women (women presenting at least one of the following genital symptoms: leucorrhoea, pelvic pain/dyspareunia, pruritus, menorrhagia, metrorrhagia, or dysuria).

This study was approved by the Institutional Review Board of Fez, Morocco (N° 02/15).

2.2. Genotypic Characterization of NG

One hundred and forty-nine NG positives samples by PCR were used to determine the types of PPNG, TRNG, and CRNG

The detection of beta-lactamase-producing plasmid types (Africa, Asia, and Toronto), tetM tetracycline resistance plasmid types (America and Dutch), and the determination of the mutation Ser-91 in gyrA was performed by PCR using specific primers followed by HinfI digestion of tetM and gyrA products as previously described [9, 11]. Primers used, PCR amplicons sizes are listed in Table 1. Restriction fragments were determined in 1.5% agarose gel electrophoresis (Table 1). Positive and negative controls were used for each PCR.

2.3. Statistical Analysis

The statistical analysis was performed using SPSS (version 20, SPSS Inc., Chicago, Illinois, USA). Descriptive analysis was performed for all variables. The relationship between different variables and TRNG, PPNG, and ciprofloxacin resistant NG was checked using Fisher exact test or chi-squared test when applicable. Multivariate analysis was conducted using a binary logistic regression model to determine the risk factors. Variables included in the model were those with p-value ≤0.20 and the odds ratios (OR) and 95% confidence intervals (CI) were calculated. In all tests, the value p <0.05 was considered statistically significant.

3. Results

3.1. Studied Population

A total of 149 NG positives samples of consenting women were included in this study. The mean age of participants was 43.16 years (20-66 years) and 27 (18.1%) were pregnant. Illiteracy rate was 61.5%. Most of women were married (91.8%). Among them, 37.9% had more than three pregnancies and nearly 31% of them had more than three children. The characteristics of the included population are presented in Table 2.

3.2. Prevalence of TRNG, PPNG, and CRNG

In this study, samples containing at least one of the plasmids-encoding resistances for penicillin or tetracycline or the substitution in GyrA (Ser-91Phe) were considered resistant. Among the 149 NG positives samples, 135 (90.1%) showed a molecular resistance profile to at least one antibiotic with the predominance of resistance to ciprofloxacin. In fact, 36 (24.2%) NG cases harbored PPNG, 69 (46.3%) cases harbored TRNG, and 116 (77.9%) cases showed mutated Ser-91 in gyrA. The double resistance was detected in 50 cases (33.6%), while the triple resistance (TRNG/PPNG/CRNG) was found in 18 samples (12.1%) as reported in Table 3.

3.3. PPNG and TRNG Genotypes

From a total of 36 PPNG isolates, the Toronto, Asian, and Toronto/Asian types were detected in 13 (36.1%), 10 (27.8%), and 13 (36.1) cases, respectively, whereas the African type was not detected. In addition, the American type of TRNG was detected in 92.8% (64/69) of cases, while the Dutch type was detected in 7.2% (5/69) of cases. The genotypic profile of samples is presented in Table 3.

3.4. Association of Beta-Lactamase-Encoding and TetM-Encoding Plasmids

A total of 22 (61.1%) samples harboring PPNG plasmid also contained the TetM-encoding plasmid. This association is statistically significant (P=0.041). However, there was no significant association between the presence of beta-lactamase encoding plasmids and the type of TRNG variant plasmid (Table 4).

3.5. Prevalence of PPNG, TRNG, and CRNG according to Consultation Motifs

The association of NG resistance mechanisms with clinical symptoms was studied. The results show that highest percentages of PPNG and TRNG resistance (94.4% and 89.9%, respectively) were obtained in nonpregnant women than in pregnant women (5.6% and 10.1%, respectively) with P value <0.05) (Table 5). However, the TRNG is statistically associated with the absence of symptoms (p = 0.017) (Table 5).

3.6. Correlation between NG Resistance and Variables

The association of demographics and clinical variables with NG resistance profiles was studied in a univariate analysis. The results were reported in Tables 6 and 7.

All variables with p ≤ 0.20 were used in a step wise selection procedure in logistic regression analysis. In the final model, the obtained results show that literate women and those with high number of parity (>3) were at higher risk of TRNG (OR 95% IC1.623 [1.180–2.231]) and 2.920 [1.303–6.547], respectively). Those living in urban area and married ones seem to be at high risk of CRNG (p<0.05) compared to the others. However, literate patients were at higher risk of infection with species harboring two different PPNG and carrying triple drug resistance (PPNG/TRNG/CRNG) (Table 8).

4. Discussion

Gonococcal infection represents a major public health issue [13]. In fact, its extent of genetic recombination leads to a large phenotypic diversity that enhances the transmission and ability to escape the host immune systems [14]. This tendency for genetic transformation and recombination also leads to the rapid spread of antibiotics resistance genes in many countries of the world. This includes resistance to sulfonamides, macrolides, penicillins, tetracyclines, and fluoroquinolones [3]. These resistances are related in part to a Ser-91Phe substitution in gyrA for ciprofloxacin (Fluoroquinolones) resistant gonococcal strains, a tetM gene for plasmid-mediated tetracycline resistance (TRNG), and a penicillinase encoding plasmids (Asian, African, Toronto, Rio, Nîmes, New Zealand, and Johannesburg) for plasmid-mediated penicillin resistance (PPNG). However, the mutations in folP [encoding the sulfonamide target the enzyme dihydropteroate synthase (DHPS)] are linked to sulphonamide resistance and the mtrR mutations or erm genes to macrolides resistance [15].

In Morocco and especially in Fez regions area, the rate of NG infection recently detected based on molecular diagnosis was 14% (data not published). This rate is considered very high compared to the prevalence noted in the African regions (2.3%) [16]. Despite this, there is no information on the extent and mechanisms of gonorrhea resistance from this area.

This study provides the first data on PPNG, TRNG, and gyrA status in Morocco and helps to estimate the NG resistance rates. In fact, PPNG was firstly reported in United Kingdom and Thailand in 1976 [17] and TRNG was initially found in the United States in 1985 [18] and their frequency has increased over time.

In our study, PPNG was identified in 24.2% NG positive samples. This rate is similar to that detected in South Africa (25.8%) in 2010 [6], but higher than the rates observed in Rio de Janeiro (8.6%) [19] and in Manaus (14.5%) [20]. However, it still lower than that found in some Asian countries like Indonesia (60% from Bandung and 62.1% from Jakarta in 2003) [21], Bhutan (86% from 2007 to 2011) [4], and Thailand (82.1% in 2012) [22]. Comparisons between these results and the study results must be interpreted with caution since the epidemiology of resistant isolates differs in the time and the detected rates were determined in different years and in populations with different risk factors.

The PPNG were genotyped and the results showed that Toronto and Asian types were detected separately or in combination, whereas the African type was not detected. Unfortunately, data on PPNG types circulating in neighboring North African countries are not available and the only African data that exists are those reported in 2010 from South Africa who showed different distribution types with predominance of Toronto (44.4%) and African (35.2%) types and absence of Asian type [6]. However, during the same period (2010), the African type was the most prevalent in some Asian countries like Bangkok, Thailand, Japan, and Bangladesh [22–24]. Even if resistance profiles distribution can change over the time, the present results let us suppose that the circulating isolates, in a given period of time, are different according to geographical area.

Samples with two PPNG types (Toronto/Asian) were present with high frequency (36.1%). This can be explained by the fact that the infecting strains harbor two plasmids or by mixed gonococcal infection. This last hypothesis seems more plausible since (1) the presence of two plasmids with the same origin of replication is unusual and (2) mixed gonococcal infection at the same anatomical site has been documented and proven by culture in several studies [25–28]. In fact, those studies reported different rates of mixed infections according to studied population (1.3%-3.2% in Australia and 40% in sex worker, USA). The results of the present study give an evidence of the high rate of mixed infection and show that PPNG typing can be helpful in their detection. It also shows the advantage of molecular techniques used directly on clinical samples, for epidemiological antimicrobial resistance (AMR) surveillance.

The NG mixed infections can be explained by sexual behaviors of patients and/or their partners. In fact, individuals with multiple sexual partners are at a higher risk of mixed NG infections and risk is higher in areas with a high incidence of gonococcal infections. In our study, the majority of women stated that they only had one partner. In this case, simultaneous transmission of mixed strains by one partner that has been documented by Matin and Ison [27] can explain the mixed infection. To verify this hypothesis, information about sexual behavior would be required from the partner and testing of the partner should be done.

The prevalence of TRNG strains (49.5%) is similar to that found in China (52.1%) in 2015 [29] and higher than those observed in some South American countries: Manaus (12.7%) [20] and Rio de Janeiro (20%) [19]. However it still lower than the rates reported in South Africa (70.3%) [6] and in Thailand (84.1%) [22] in 2010 and 2012, respectively.

In our study, we found that American type TRNG is predominant with rate of 92.8% and the Dutch type is present only in 7.2% of cases. This tetM plasmids types distribution is similar to that obtained in Africa [98.3% (59/60) American and 0.7% (1/60) Dutch type] [30], in South Africa [76% (117/154) and American type 24% (37/154) Dutch] [6], and in Europe [82% (248/306) American and 18% (58/306) Dutch type] [30]. However, it is different from that observed in North, Central, and South America [40% (4/10) American and 60% (6/10) Dutch type] [30], in Asia [100% (35/35) Dutch type; 98.7% (377/382) Dutch and 1.3% (5/382) American type], in Bangladesh [30, 31], and in China [100% (639/639) Dutch type] [29]. These findings confirmed that the American type was the most prevalent in Africa including Morocco.

Our findings show also that the most PPNG positive samples 22 (61.1%) carried also the TetM-encoding plasmid. This is in agreement with the hypothesis supposing that the tetM plasmids plays a role in the mobilization of beta-lactamase plasmids [32]. Potentially the American type tetM plasmid is effective at mobilizing the Toronto type plasmids, as proved by the high frequency of this type of penicillinase-producing plasmid in samples containing the American type tetM plasmid.

Previous studies have demonstrated that Ser-91 mutation in gyrA for NG is mainly associated with ciprofloxacin resistance [9]. Even if other mutations have been reported in ciprofloxacin resistant isolates, several studies have proved that they usually occur in parallel with gyrA mutation. Thus, the GyrA status can be used alone for ciprofloxacin susceptibility prediction [33, 34]. In our study, a total of 116 (77.9%, 116/149) isolates showed the mutation Ser-91 in gyrA extrapolating to ciprofloxacin resistance. However, high level of CRNG has been worldwide reported and especially in Asia (93.8% in 2003; 100% from Hefei) [8, 35].

The results of this study, not previously done on Moroccan samples, add to our epidemiological knowledge and reinforces that these antibiotics (penicillin, tetracycline, and ciprofloxacin) should not be used for STI management and especially for NG, in Morocco. The results reinforce the treatment change made by the Moroccan Ministry of Health [10].

In our study, we have found that the higher percentage of PPNG and TRNG resistance were detected in nonpregnant women compared to pregnant women. Also, the higher level of TRNG was associated with asymptomatic women for genital STIs. The fact that NG causes only mildly symptomatic or asymptomatic infections, especially in women, increases the probability of disease progression and TRNG resistant isolates transmission [36] especially when the diagnosis is not routinely performed. Therefore, it is necessary to make a systematic screening for this infection.

The determination of risk factors may help in patient management and future prevention strategies. In our study, literate patients and those with high parity have a significant 2.8-fold (95%, CI, 1.295–6.045) and 2.9-fold (95%, CI, 1.303–6.547), respectively, increased risk of TRNG in comparison with illiterate women and those with ≤3 parities. Besides, there was a higher risk of infection with CRNG for married women living in urban areas than it for divorced or widowed living in rural area. However, the risk of being infected by two PPNG plasmids types and possessing the triple drug resistance was 4.5-fold (95%, CI, 1.059–19.407) and 2.8-fold (95%, CI, 1.041–7.907), respectively, higher in literate patients than in illiterate women.

Those results indicate that literate women, married women, those living in urban area, and those with high number of parity seem to be at higher risk to be infected with resistant NG. The risk determined for literate women and those living in urban area can be explained by (i) self-medication (more easily and widely used by literate people), leading to the misuse of antibiotics and therefore the resistance development; (ii) easy access to pharmacies and antibiotics (without a medical prescription) in urban area.

The risk determined for married patients and those with high parity can be explained by the fact that those women are usually sexually active and the probability of invasive acts related to delivery.

Further studies are needed to understand the influence of behavioral and socioeconomic factors on the resistance of NG to specific antibiotics such as tetracycline, penicillin, and ciprofloxacin.

This study has some limitations. In fact, low number of samples was used and only few genes were targeted to determine the resistance rates in the absence of phenotypic characterization. Nevertheless, having an estimation of NG resistance is useful for treatment strategy, especially in region where bacterial culture and characterization are not performed and patients are generally not diagnosed for STIs.

5. Conclusion

In summary, this study highlights the high rate of NG Moroccan isolates resistance to antimicrobials previously recommended for the treatment of gonorrhea (penicillin, tetracycline, and ciprofloxacin). The high rate of mixed infections shows the failure of NG and STI management. Thus, the use of molecular tools for STIs diagnosis and antimicrobial resistance detection can help in their management.

Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

Ethical Approval

This study was approved by the Institutional Review Board of Fez, Morocco (N° 02/15).

Conflicts of Interest

The authors declare that there are no conflicts of interest regarding the publication of this paper.

Acknowledgments

The authors would like to thank the staff of the Department of Obstetrics and Gynecology and the Laboratory of Anatomy-Pathology of University Hospital of Fez for their help in the collection and specimens’ preparation.

References

L. Newman, J. Rowley, S. Vander Hoorn et al., “Global estimates of the prevalence and incidence of four curable sexually transmitted infections in 2012 based on systematic review and global reporting,” PLoS ONE, vol. 10, no. 12, Article ID e0143304, 2015.
View at: Publisher Site | Google Scholar
A. Gewirtzman, L. Bobrick, K. Conner, and S. K. Tyring, “Epidemiology of Sexually Transmitted Infections,” in Sexually Transmitted Infections and Sexually Transmitted Diseases, pp. 13–34, Springer Berlin Heidelberg, Berlin, Germany, 2011.
View at: Publisher Site | Google Scholar
D. A. Lewis, “Global resistance of Neisseria gonorrhoeae,” Current Opinion in Infectious Diseases, vol. 27, pp. 62–67, 2014.
View at: Publisher Site | Google Scholar
S. Sethi, D. Golparian, M. Bala et al., “Antimicrobial susceptibility and genetic characteristics of Neisseria gonorrhoeae isolates from India, Pakistan and Bhutan in 2007-2011,” BMC Infectious Diseases, vol. 13, no. 1, 2013.
View at: Google Scholar
F. Pagotto, A.-T. Aman, L.-K. Ng, K.-H. Yeung, M. Brett, and J.-A. R. Dillon, “Sequence analysis of the family of penicillinase-producing plasmids of Neisseria gonorrhoeae,” Plasmid, vol. 43, no. 1, pp. 24–34, 2000.
View at: Publisher Site | Google Scholar
S. A. Fayemiwo, E. E. Müller, L. Gumede, and D. A. Lewis, “Plasmid-mediated penicillin and tetracycline resistance among neisseria gonorrhoeae isolates in South Africa: Prevalence, detection and typing using a novel molecular assay,” Sexually Transmitted Diseases, vol. 38, no. 4, pp. 329–333, 2011.
View at: Publisher Site | Google Scholar
D. M. Gascoyne-binzi, J. Heritage, and P. M. Hawkey, “Nucelotide sequences of the tet(m) genes from the american and dutch type tetracycline resistance plasmids of neisseria gonorrhoeae,” Journal of Antimicrobial Chemotherapy, vol. 32, no. 5, pp. 667–676, 1993.
View at: Publisher Site | Google Scholar
Y. Chen, Y. Gong, T. Yang et al., “Antimicrobial resistance in Neisseria gonorrhoeae in China: a meta-analysis,” BMC Infectious Diseases, vol. 16, no. 1, 2016.
View at: Publisher Site | Google Scholar
M. Tanaka, H. Nakayama, M. Haraoka, T. Saika, I. Kobayashi, and S. Naito, “Antimicrobial resistance of Neisseria gonorrhoeae and high prevalence of ciprofloxacin-resistant isolates in Japan, 1993 to 1998,” Journal of Clinical Microbiology, vol. 38, no. 2, pp. 521–525, 2000.
View at: Google Scholar
A. Hançali, F. Ndowa, B. Bellaji et al., “Antimicrobial resistance monitoring in Neisseria gonorrhoeae and strategic use of funds from the Global Fund to set up a systematic Moroccan gonococcal antimicrobial surveillance programme,” Sexually Transmitted Infections, vol. 89, no. 4, pp. iv24–iv27, 2013.
View at: Publisher Site | Google Scholar
R. Lawung, R. Cherdtrakulkiat, A. Charoenwatanachokchai, S. Nabu, W. Suksaluk, and V. Prachayasittikul, “One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae,” Journal of Microbiological Methods, vol. 77, no. 3, pp. 323–325, 2009.
View at: Publisher Site | Google Scholar
S. R. Lee, J. M. Chung, and Y. G. Kim, “Rapid one step detection of pathogenic bacteria in urine with sexually transmitted disease (STD) and prostatitis patient by multiplex PCR assay (mPCR),” The Journal of Microbiology, pp. 45–453, 2007.
View at: Google Scholar
J. W. Tapsall, F. Ndowa, D. A. Lewis, and M. Unemo, “Meeting the public health challenge of multidrug- and extensively drug-resistant,” Expert Review of Anti-infective Therapy, vol. 7, no. 7, pp. 821–834, 2014.
View at: Publisher Site | Google Scholar
H. L. Hamilton and J. P. Dillard, “Natural transformation of Neisseria gonorrhoeae: from DNA donation to homologous recombination,” Molecular Microbiology, vol. 59, no. 2, pp. 376–385, 2006.
View at: Publisher Site | Google Scholar
M. Unemo and W. M. Shafer, “Antimicrobial resistance in Neisseria gonorrhoeae in the 21st Century: past, evolution, and future,” Clinical Microbiology Reviews, vol. 27, no. 3, pp. 587–613, 2014.
View at: Publisher Site | Google Scholar
World Health Organization and Department of Reproductive Health and Research, “Global incidence and prevalence of selected curable sexually transmitted infections: 2008,” Reproductive Health Matters, vol. 20, no. 40, pp. 207–209, 2012.
View at: Publisher Site | Google Scholar
A. Percival, J. Rowlands, J. E. Corkill et al., “Penicillinase producing gonococci in Liverpool,” The Lancet, vol. 2, no. 8000, pp. 1379–1382, 1976.
View at: Publisher Site | Google Scholar
J. S. Knapp, J. M. Zenilman, W. James et al., “Frequency and distribution in the united states of strains of neisseria gonorrhoeae with plasmid-mediated, high-level resistance to tetracycline,” The Journal of Infectious Diseases, vol. 155, no. 4, pp. 819–822, 1987.
View at: Publisher Site | Google Scholar
N. A. Barreto, R. R. Santanna, L. B. Silva, A. A. Uehara, R. C. Guimarães, I. M. Duarte et al., “Phenotypic and molecular characterization of Neisseria gonorrhoeae isolated in Rio de Janeiro, Brazil, 2002-2003,” in DST - Jornal brasileiro de doenças sexualmente transmissiveis, vol. 16, pp. 32–42, 2004.
View at: Google Scholar
W. A. Ferreira, W. S. Vasconcelos, J. S. Gomes et al., “Neisseria Gonorrhoeae Antimicrobial Resistance in Manaus: 2005-2006 Period,” DST - Jornal brasileiro de doenças sexualmente transmissiveis, vol. 19, pp. 175-176, 2007.
View at: Google Scholar
M. Ieven, M. Van Looveren, S. Sudigdoadi et al., “Antimicrobial susceptibilities of Neisseria gonorrhoeae strains isolated in Java, Indonesia,” Sexually Transmitted Diseases, vol. 30, no. 1, pp. 25–29, 2003.
View at: Publisher Site | Google Scholar
R. Lawung, R. Cherdtrakulkiat, A. Charoenwatanachokchai, S. Nabu, S. Lokpichart, and V. Prachayasittikul, “Antimicrobial resistance markers as a monitoring index of gonorrhoea in Thailand,” Acta Microbiologica et Immunologica Hungarica, vol. 59, no. 2, pp. 157–169, 2012.
View at: Publisher Site | Google Scholar
S.-I. Nakayama, C. Tribuddharat, S. Prombhul et al., “Molecular analyses of TEM genes and their corresponding penicillinase-producing Neisseria gonorrhoeae isolates in Bangkok, Thailand,” Antimicrobial Agents and Chemotherapy, vol. 56, no. 2, pp. 916–920, 2012.
View at: Publisher Site | Google Scholar
M. Ohnishi, E. Ono, K. Shimuta, H. Watanabe, and N. Okamura, “Identification of TEM-135 -Lactamase in Penicillinase-Producing Neisseria gonorrhoeae Strains in Japan,” Antimicrobial Agents and Chemotherapy, vol. 54, no. 7, pp. 3021–3023, 2010.
View at: Publisher Site | Google Scholar
N. Goire, R. Kundu, E. Trembizki et al., “Mixed gonococcal infections in a high-risk population, Sydney, Australia 2015: Implications for antimicrobial resistance surveillance?” Journal of Antimicrobial Chemotherapy, vol. 72, no. 2, pp. 407–409, 2017.
View at: Publisher Site | Google Scholar
F. Lynn, M. M. Hobbs, J. M. Zenilman et al., “Genetic typing of the porin protein of Neisseria gonorrhoeae from clinical noncultured samples for strain characterization and identification of mixed gonococcal infections,” Journal of Clinical Microbiology, vol. 43, no. 1, pp. 368–375, 2005.
View at: Publisher Site | Google Scholar
I. M. Martin and C. A. Ison, “Detection of mixed infection of Neisseria gonorrhoeae,” Sexually Transmitted Infections, vol. 79, no. 1, pp. 56–58, 2003.
View at: Publisher Site | Google Scholar
E. Trembizki, C. Doyle, C. Buckley et al., “Estimating the prevalence of mixed-type gonococcal infections in Queensland, Australia,” Sexual Health, vol. 12, no. 5, pp. 439–444, 2015.
View at: Publisher Site | Google Scholar
H. Zheng, X. Wu, J. Huang et al., “The prevalence and epidemiology of plasmid-mediated penicillin and tetracycline resistance among Neisseria gonorrhoeae isolates in Guangzhou, China, 2002–2012,” BMC Infectious Diseases, vol. 15, no. 1, 2015.
View at: Publisher Site | Google Scholar
A. Turner, K. R. Gough, and J. P. Leeming, “Molecular epidemiology of tetM genes in Neisseria gonorrhoeae,” Sexually Transmitted Infections, vol. 75, no. 1, pp. 60–66, 1999.
View at: Publisher Site | Google Scholar
M. A. Alam, Z. U. Chowdhury, A. H. Chowdhury, and M. Rahman, “Epidemic plasmids in Neisseria gonorrhoeae isolated from high-risk population in Bangladesh.,” Mymensingh medical journal : MMJ, vol. 21, no. 2, pp. 220–225, 2012.
View at: Google Scholar
S. K. Sarafian, C. A. Genco, M. C. Roberts, and J. S. Knapp, “Acquisition of β-lactamase and TetM-containing Conjugative Plasmids by Phenotypically Different Strains of Neisseria gonorrhoeae,” Sexually Transmitted Diseases, vol. 17, no. 2, pp. 67–71, 1990.
View at: Publisher Site | Google Scholar
M. J. Siedner, M. Pandori, L. Castro et al., “Real-time PCR assay for detection of quinolone-resistant Neisseria gonorrhoeae in urine samples,” Journal of Clinical Microbiology, vol. 45, no. 4, pp. 1250–1254, 2007.
View at: Publisher Site | Google Scholar
Y. Yang, “Antimicrobial susceptibility and molecular determinants of quinolone resistance in Neisseria gonorrhoeae isolates from Shanghai,” Journal of Antimicrobial Chemotherapy, vol. 58, no. 4, pp. 868–872, 2006.
View at: Publisher Site | Google Scholar
F.-X. Jiang, Q. Lan, W.-J. Le, and X.-H. Su, “Antimicrobial susceptibility of Neisseria gonorrhoeae isolates from Hefei (2014-2015): Genetic characteristics of antimicrobial resistance,” BMC Infectious Diseases, vol. 17, no. 1, article no. 366, 2017.
View at: Publisher Site | Google Scholar
R. C. Brunham, F. A. Plummer, and R. S. Stephens, “Bacterial antigenic variation, host immune response, and pathogen-host coevolution,” Infection and Immunity, vol. 61, no. 6, pp. 2273–2276, 1993.
View at: Google Scholar

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Copyright © 2018 Safae Karim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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