Infectious Diseases in Obstetrics and Gynecology / 2019 / Article / Tab 3

Research Article

Accuracy of Curable Sexually Transmitted Infections and Genital Mycoplasmas Screening by Multiplex Real-Time PCR Using a Self-Collected Veil among Adult Women in Sub-Saharan Africa

Table 3

Two-by-two tables of cervicovaginal specimens self-collected using the V-Veil-Up Gyn Collection Device (V-Veil-Up Pharma Ltd.) compared to clinician-collected endocervical swab specimens for the detection by multiplex real-time PCR of genital bacteria, including common curable sexually transmitted infections (group I) and commensal mycoplasmas (group II) among 251 adult women living in N’Djamena, Chad, and recruited from the community.

All genital bacteriaGroup IGroup II

Clinician-collected swab specimenClinician-collected swab specimenClinician-collected swab specimen

Positive (N=134)Negative (N=117)Positive (N=6)Negative (N=245)Positive (N=134)Negative (N=117)

Veil- based
self-collected specimen
Positive (N=144)13014Positive (N=7)61Positive (N=144)13014
Negative (N=107)4103Negative (N=244)0244Negative (N£=107)4103

Estimate95 CIEstimate95 CIEstimate95 CI

Sensitivity ()97.0[92.5-99.2]NA+-97.0[92.5-99.2]

Specificity ()88.0[80.7-93.3]NA+-88.0[80.7-93.3]

Agreement ()92.899.692.8

Cohen’s κ coefficient0.850.920.85

Total number of veil based-self collected specimens positive for all genital microorganisms.
Total number of veil based-self collected specimens negative for all genital microorganisms.
Total number of veil based-self collected specimens positive for genital microorganisms of Group I.
Total number of veil based-self collected specimens negative for genital microorganisms of Group I.
Total number of veil based-self collected specimens positive for genital microorganisms of Group II.
£Total number of veil based-self collected specimens negative for genital microorganisms of Group II.
The Cohen’s κ coefficient was interpreted according the Landis and Koch scale [32]: For κ value 0, the agreement is considered to be less than what would be expected by chance; for κ values 0.01–0.20, only a “slight agreement” is present; for κ values 0.21–0.40, the agreement is considered to be “fair”; for κ values 0.41–0.60, the agreement is said to be “moderate”; for κ values 0.61–0.80, the agreement is considered “good”; and finally, for κ values 0.81–0.99, the agreement is said to be “almost perfect”.
+NA: not attributable (because of the low common STIs positivity rate (<3%) in study population which makes impossible to calculate an accurate estimate of sensitivity and specificity for the group I).

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.