Research Article

Effects of mti-2 Transgenic Potato Plants on the Aphid Myzus persicae (Sternorrhyncha: Aphididae)

Table 1

Average (± s.d.) trypsin activity in presence of leaf protein crude extracts from nontransformed (NT) and mti-2-expressing potato lines.

LineAverage trypsin activity  (pmol hydrolysed azocaseine/min/mg of trypsin)

NT (= 100% activity) 9 3 . 0 ± 0 . 2
DM1 8 3 . 7 ± 0 . 0
DM2 7 5 . 1 ± 1 0 . 2
DM3 7 9 . 0 ± 1 . 8
DM4 7 9 . 5 ± 6 . 7
DM5 6 9 . 8 ± 5 . 5
DM 6 6 0 . 2 ± 1 0 . 3
DM 7 4 8 . 6 ± 4 . 1
DM8 8 1 . 8 ± 1 . 4
DM9 6 6 . 6 ± 0 . 3
DM1 1 6 3 . 2 ± 0 . 1
DM12 1 0 0 . 2 ± 1 . 6
DM13 7 7 . 9 ± 4 . 5
DM14 7 3 . 8 ± 3 . 7
DM15 7 4 . 4 ± 4 . 8
DM16 7 5 . 0 ± 9 . 9
DM17 7 3 . 1 ± 3 . 2
DM18 8 2 . 1 ± 3 . 9
DM1 9 6 0 . 7 ± 9 . 4
DM20 6 6 . 9 ± 3 . 2
DM21 8 0 . 2 ± 3 . 5
DM22 7 5 . 8 ± 0 . 8
DM23 7 8 . 8 ± 8 . 1
DM24 8 0 . 3 ± 1 1 . 4
DM26 7 1 . 7 ± 2 . 9
DM28 8 0 . 3 ± 4 . 0
DM29 6 8 . 6 ± 0 . 1
DM30 7 3 . 0 ± 5 . 3
DM31 8 5 . 1 ± 1 1 . 3
DM32 7 6 . 4 ± 1 3 . 2
DM33 8 3 . 1 ± 5 . 7
DM34 6 7 . 7 ± 7 . 0
DM35 8 3 . 2 ± 0 . 1

Selected lines in which at least 30% of trypsin activity were inhibited compared to nontransformed line. Trypsin activity inhibition could not be determined for DM10, DM25, and DM27 lines.