Genetic Transformation of Common Bean (Phaseolus vulgaris L.) with the Gus Color Marker, the Bar Herbicide Resistance, and the Barley (Hordeum vulgare) HVA1 Drought Tolerance Genes

Table 1

Transient expression of GUS using different gene gun pressure (psi), DNA plasmid concentration and bombardment frequency for optimizing the Biolistic bombardment conditions for the pCATIF containing the gus gene.

Bombardment pressure (psi)	Concentration of plasmid DNA (μg)	Bombardment frequency	Mean transformation percent
500	1.5	1	0.1±0.04
500	1.5	2	0.2±0.10
500	1.5	3	0.4±0.30
500	3	1	0.1±0.04
500	3	2	0.6±0.32
500	3	3	0.7±0.32
1000	1.5	1	2.9±0.67
1000	1.5	2	3.9±1.4
1000	1.5	3	5.1±1.2
1000	3	1	5.6±1.0
1000	3	2	8.1±0.3
1000	3	3	7.4±1.0
1100	1.5	1	7.2±0.70
1100	1.5	2	8.4±0.74
1100	1.5	3	8.2±0.50
1100	3	1	7.5±0.69
1100	3	2	4.8±0.93
1100	3	3	3.3±0.92