Research Article | Open Access
Julie A. Blessitt, Salliana R. Stetina, Ted P. Wallace, Peggy T. Smith, Gabriel L. Sciumbato, "Cotton (Gossypium hirsutum) Cultivars Exhibiting Tolerance to the Reniform Nematode (Rotylenchulus reniformis)", International Journal of Agronomy, vol. 2012, Article ID 893178, 8 pages, 2012. https://doi.org/10.1155/2012/893178
Cotton (Gossypium hirsutum) Cultivars Exhibiting Tolerance to the Reniform Nematode (Rotylenchulus reniformis)
A field study was conducted over a two-year period (2006-2007) at the Delta Research and Extension Center, Stoneville, MS, USA to screen selected entries in the 2006 Mississippi Cotton Variety Trials for tolerance to the reniform nematode (Rotylenchulus reniformis). Trials were conducted in nonirrigated fields with primarily sandy loam soils. Though some variability was noted between test locations and years, six of 13 cotton (Gossypium hirsutum) cultivars tested were considered tolerant to the reniform nematode: “Cropland Genetics 3520 B2RF,” “DynaGrow 2520 B2RF,” “Stoneville 5242 BR,” “Stoneville 5599 BR,” “Deltapine 488 BG/RR,” and “Fibermax 960 B2R.” Of these, the first three exhibited yields similar to the productive cultivar “Deltapine 445 BG/RR” in all environments. Though they will not suppress the reniform nematode population, these cultivars can help reduce economic losses attributed to this pathogen in the Midsouth region of the USA.
When planting any crop, it is important to consider potential losses due to susceptibility to pests. Upland cotton (Gossypium hirsutum) is an important crop in Mississippi. In 2008, cotton losses in Mississippi attributed to nematodes were at 10% (81,250 bales), the majority (8%) due to the reniform nematode (Rotylenchulus reniformis), with an approximate loss of 65,000 bales . This estimate ranks reniform nematode as the most damaging nematode threat to cotton in the state.
Rotation to nonhost crops and application of nematicides have been used for reniform nematode population management. Rotation to a non-host crop reduces the nematode population in the soil [2–4], though the population may rebound quickly when a susceptible crop is planted the following cropping season . Management through chemical treatment of infested soils remains a useful control practice for reniform nematode, though use of nematicides is often cost prohibitive. Nematicides are dangerous to handle, can be fatal, pose a risk to ground water, and are toxic to wildlife . These concerns have resulted in a gradual reduction in the number of products available, including discontinuation of the popular nematicide aldicarb . If highly susceptible cultivars could be avoided in nematode-infested areas and replaced with tolerant or resistant cultivars, some of the risks associated with nematicide use could be avoided.
Unfortunately, no commercial cotton cultivars with resistance to reniform nematode exist [3, 8]. It will likely be years before novel genes for resistance recently identified in the related Gossypium species G. longicalyx , G. aridum , and G. barbadense  are available to growers.
The absence of resistance has shifted some focus to finding tolerance to the reniform nematode in G. hirsutum. Tolerance can be defined as the “ability of a plant to sustain the effects of a disease without dying or suffering serious injury or crop loss” . Tolerant plants support reproduction by nematodes while sustaining lower yield losses than other cultivars having comparable levels of nematode infection . Therefore, identification of tolerance requires an analysis and comparison of cultivars for yield performance under field conditions [13–21].
In previous years, researchers from several labs attempting to identify reniform nematode tolerant cultivars have had very limited success. Usery et al.  saw some possible tolerance in two upland cotton cultivars, Stoneville 4793 and Suregrow 521R, but their results were inconsistent across years and locations. Cook et al.  identified possible tolerance in only a few high yielding breeding lines tested. Stetina et al.  identified three possible tolerant cultivars (“Suregrow 215 BR,” “Paymaster 1218 BR,” and “Deltapine 449 BR”) out of the 39 lines tested, but suggested that tolerant lines, in general, were not widely available. Germplasm lines characterized as having reniform nematode tolerance have been released [15, 22, 23]. However, as most commercial cultivars have a relatively short “shelf life,” there is a continual need to evaluate new commercial cultivars and germplasm releases for tolerance.
This study was undertaken to identify cotton cultivars that exhibit tolerance to the reniform nematode in the Mississippi Delta. Though tolerant cultivars will not help manage nematode populations in the long term, they could help minimize economic losses in the short term. Readily available, tolerant cultivars could provide immediate relief for a growing economic problem until a time when true resistance becomes available in commercial cultivars.
2. Materials and Methods
Field experiments were conducted during the 2006 and 2007 growing seasons to evaluate selected cultivars from the 2006 Mississippi Cotton Variety Trials  for tolerance to the reniform nematode. Fields selected for this study had a history of continuous cotton for several years and were characterized as having a high natural infestation of reniform nematodes. In the fall prior to planting, reniform nematode populations met or exceeded the treatment threshold level of 16.2/cm3 soil [25–27]. Experiments were conducted at four different sites at the Delta Research and Extension Center (DREC), Stoneville, MS. In 2006, experiments were conducted in Field 4 and the Barn Field, and in 2007 the experiments were located in Field 1 and Field 12.
Although water was available, supplemental irrigation was not required. Seasonal precipitation and soil temperature (10 cm depth) data were obtained from the Delta Agricultural Weather Center at Mississippi State University (http://www.deltaweather.msstate.edu/historic_ag_weather_data/historic_ag_weather_data.htm). Rainfall amounts and seasonal patterns during the 2006 growing season differed from those observed in 2007 (Figure 1). Less rain fell during 2006, particularly early in the season. In contrast, 2007 had more precipitation and abundant rainfall during July. Minimum (Figure 2) and maximum (Figure 3) soil temperatures varied little between years.
According to the United States Department of Agriculture Natural Resources Conservation Service (http://websoilsurvey.nrcs.usda.gov/app/WebSoilSurvey.aspx), Field 4 consisted of a Bosket very fine sandy loam (Alfisols Udalfs Hapludalfs Mollic; fine loamy, mixed, active, thermic) and a Commerce series soil (Inceptisols Aquepts Endoaquepts Fluvaquentic; fine silty, mixed, superactive, nonacid, thermic). Field 1 was a Bosket very fine sandy loam. Field 12 consisted of Beulah very fine sandy loam (Inceptisols Udepts Dystrudepts Typic; coarse loamy mixed, active, thermic), Bosket very fine sandy loam, and Commerce series soils. The Barn Field contained both Bosket series and Commerce series soils.
Reniform nematode distribution is not necessarily uniform across a field, so experiments were arranged in a split-plot design to increase precision in measuring effects of the nematicide (subplot treatment) on crop yield. The main plot consisted of 13 cotton cultivars. Eleven were chosen at random to represent the major seed companies participating in the 2006 Mississippi Cotton Variety Trials : “Croplan Genetics 3520 B2RF,” “Deltapine 445 BG/RR,” “Deltapine 449 BG/RR,” “Deltapine 455 BG/RR,” “Deltapine 488 BG/RR,” “DynaGrow 2520 B2RF,” “Fibermax 960 B2R,” “Phytogen 370 WR,” “Phytogen 485 WRF,” “Stoneville 5242 BR,” and “Stoneville 5599 BR.” Also included were cultivars Deltapine 20B, which supported high levels of reniform nematode reproduction in previous tests , and DES119, an obsolete conventional cultivar considered tolerant to reniform nematode . Commercial cultivar Deltapine 445 BG/RR was designated as the check cultivar for yield based upon its superior performance in the 2005 Mississippi Cotton Variety Trials . All cultivars were developed and have been marketed for the growing conditions represented by DREC trial sites. Subplots consisted of nontreated plots and nematicide-treated plots receiving aldicarb (Temik 15 G, Bayer CropScience, Durham, NC) at 0.84 kg of active ingredient per hectare (a.i./ha) applied in furrow at planting followed by a side-dress application of 1.17 kg a.i./ha at pinhead square. The application of aldicarb at planting time under ideal conditions is sufficient to keep reniform nematode levels manageable for approximately six weeks . The subplot treatment allowed for determination of nematode reproduction values and provided a basis for comparison of yields in nematicide-treated versus nontreated plots within cultivars for interpolation of tolerance.
Main plots were replicated 5 times in each field in a randomized complete block arrangement. Main plots consisted of eight 12.2 m rows spaced 1 m apart with 13 seed planted per row meter using a 4-row cone plot-type Almaco planter (Almaco, Nevada, IA). Subplots were four rows. Prior to planting, trifluralin (Trifluralin 4 EC, Albaugh, Inc., Ankeny, IA; 1.2 L/ha) was applied to all fields for weed control. After planting, a combination of cultivation and herbicides recommended for use in conventional cotton in Mississippi was used as needed to manage weeds. Herbicides used included prometryn (Caparol 4 L, Syngenta Crop Protection, Inc., Greensboro, NC), fluometuron (Cotoran 4 L, Makhteshim Agan of North America, Inc., Raleigh, NC), MSMA (MSMA 6.6, Drexel Chemical Company, Memphis, TN), lactofen (Cobra 2 EC, Valent U.S.A. Corporation, Walnut Creek, CA), pyrithiobac sodium (Staple LX, DuPont Crop Protection, Wilmington, DE), 2-pyridinesulfonamide (Envoke, Syngenta Crop Protection, Inc., Greensboro, NC), and paraquat dichloride (Gramoxone Inteon, Sygenta Crop Protection, Inc., Greensboro, NC). Insecticides, defoliants, and growth regulators were applied as needed according to standard production guidelines for Mississippi. Due to the lack of an in-furrow insecticide in the nontreated subplots, early-season thrips were managed with acephate (Orthene 90 S, Valent U.S.A. Corporation, Walnut Creek, CA; 0.2 kg/ha). Dates of importance pertaining to planting, harvest, nematicide application, and sampling are shown in Table 1.
Ten soil cores (2.5 cm in diameter and 25.4 cm deep) were collected randomly from the center two rows of each subplot at planting, midseason, and harvest. Samples were stored at 13°C until extraction , with an understanding of some nematode population loss during storage . Reniform nematodes were extracted from 200 cm3 of soil by Seinhorst elutriation , followed by sucrose centrifugation using a 454 g/L sugar solution . Reniform nematodes per liter of soil were calculated after counting the nematodes in one-sixth of a partitioned petri dish with a stereomicroscope.
Cotton plots were harvested with a Case 2022 spindle-type picker (Case International Harvester, Racine, WI) customized for harvesting test plots. The center two rows of each 4-row plot were harvested and weighed.
Data were evaluated to determine possible interactions between field, cultivar, and nematicide. Field, a variable that included both seasonal and location differences, was considered a random-effect parameter while testing all possible interactions of fixed effects. Assuming field effect was random allowed inferences over a range of experimental conditions . Data were combined across fields only when no statistically significant interactions involving fields were detected. Statistical analysis was completed using the PROC MIXED procedure of SAS (SAS Institute Inc., Cary, NC). Means separation using differences of least squares means was accomplished using the macro PDMIX800  to convert mean separation output to letter groupings. Replications (nested within field) and associated interactions were considered random effects. Tolerance indices were analyzed with fixed effects of cultivar.
Tolerant cultivars are defined as those that yield well even while supporting reniform nematode reproduction. Therefore, reproductive indices (RIs) were calculated for each subplot asRI = Pf/Pi, where,Pf = final population andPi = initial population
over a given time period. To analyze population changes for early and late season periods, late season RI is calculated using Pf/Pm (final population/midseason population), where Pm is substituted for Pi. Early season RI is calculated using Pm/Pi (midseason population/initial population) [37, 38]. RI values greater than 1.0 indicate that the nematode population is increasing in that plot.
Differences in seed cotton yield between nematicide-treated and -nontreated plots were determined using ANOVA. For plots that did not experience an increase in yield from the nematicide application, tolerance to the reniform nematode was further described in terms of a tolerance index (TI) as reported by Koenning et al. . The TI describes cotton yield response to nematicide application in plots infested with reniform nematode, where TI values increase as tolerance increases. The TI is calculated for each cultivar as
3.1. Treatment Effects
The significance levels for main effects and their interactions are given in Table 2 for early and late season RI, seed cotton yield, and TI. Differences among cultivars with respect to early season RI were significant at and differences in TI were significant at . Significant effects of nematicide treatment on early season RI () were noted. Significant differences among cultivars and nematicide treatments were observed for seed cotton yield (), though responses were influenced by interactions () with the random effect of field. Late season RI was not significantly affected by either cultivar or nematicide. Differences () among fields were detected for all parameters, though this variation was considered a random effect.
vFixed effects are the main effects of cultivar (C) and nematicide and all interactions of those main effects; random variables are field (), , , and . |
wEarly season RI = Pm/Pi = (midseason reniform nematode count)/(planting reniform nematode count).
xLate season RI = Pf/Pm = (harvest reniform nematode count)/(planting reniform nematode count).
ySeed cotton yield taken from the middle two rows of subplots.
zTI = [(seed cotton yield in nontreated plot)/(seed cotton yield in nematicide-treated plot)]*100.
3.2. Differences among Cultivars
Early season RI values were highest for “Deltapine 445 BG/RR” (high yield check cultivar), “Deltapine 449 BG/RR,” and “Stoneville 5242 BR” (Table 3), though only the early season RI for “Deltapine 449 BG/RR” was significantly higher than the RI on the other 10 cultivars in the test. Although “Phytogen 370 WR” produced the lowest early season RI, it differed significantly only from “Deltapine 449 BG/RR” and “Stoneville 5242 BR.” Differences among cultivars were not observed for late season RI, with values ranging from 1.29 for “Deltapine 449 BG/RR” to 4.18 for “Stoneville 5599 BR.” All RI values were greater than 1.0, indicating that all cultivars supported reniform nematode reproduction.
w Early season RI = Pm/Pi = (midseason reniform nematode count)/(planting reniform nematode count). |
xMeans followed by the same letter in the same column are not significant at the 0.05 level based on differences of least squares means.
yControl cultivar with respect to yield.
Although seed cotton yields varied among cultivars, response for yield depended upon the field in which the trial was conducted (Table 4). In general, yields tended to be lower in 2006 (Field 4 and Barn Field) compared to in 2007 (Fields 1 and 12). No differences in cultivars were detected for yield in the Barn Field. In the remaining three fields, “Croplan Genetics 3520 B2RF,” “Phytogen 370 WR,” “Phytogen 485 WRF,” and “Stoneville 5242 BR” tended to rank among the highest yielding entries whereas “DES 119” and “Fibermax 960 B2R” tended to rank among the lowest yielding entries.
wSeed cotton yield taken from the middle two rows of subplots. |
xMeans followed by the same letter in the same column are not significantly different at the 0.05 level based on differences of least squares means.
yControl cultivar with respect to yield.
3.3. Differences among Nematicide Treatments
When averaged across all cultivars, the early season RI for reniform nematode was higher in plots that had not been treated with nematicides than in plots that received nematicide applications (Table 5). Seed cotton yield was higher in plots treated with nematicide (Table 5). Together, yield and RI response served as an indication that conditions conductive for evaluating tolerance had been established. However, because a significant nematicide x field effect was detected, the effect of nematicide application on yield was examined for individual fields (Table 6). No differences in yield were observed between treated and nontreated plots in Field 4, but the remaining fields all had significantly higher seed cotton yields in treated plots than in nontreated plots. Nematicide treatment did not affect late season RI (Table 2).
xEarly season RI = Pm/Pi = (midseason reniform nematode count)/(planting reniform nematode count); means followed by the same letter in this column are not significantly different at the 0.10 level based on ANOVA. |
ySeed cotton yield taken from the middle two rows of subplots; means followed by the same letter in this column are not significantly different at the 0.05 level based on ANOVA.
zAldicarb applied in furrow at planting (0.84 kg a.i./ha) plus side dress application at pinhead square (1.17 kg a.i./ha).
x Seed cotton yield taken from the middle two rows of subplots. |
y Means followed by the same letter in the same column are not significantly different at the 0.05 level based on ANOVA.
z Aldicarb applied in furrow at planting (0.84 kg a.i./ha) plus side dress application at pinhead square (1.17 kg a.i./ha).
3.4. Identification of Tolerant Cultivars
All cultivars evaluated supported reniform nematode reproduction, meeting one requirement in defining a tolerant cultivar. Seed cotton yields in nematicide-treated and nontreated subplots for each cultivar are compared in Table 7. Cultivars whose yields were equivalent in treated and nontreated plots met the second criterion in defining a tolerant cultivar. As defined, cultivars considered tolerant to reniform nematode were “Croplan Genetics 3520 B2RF,” “Deltapine 20B,” “Deltapine 488 BG/RR,” “DES 119,” “DynaGrow 2520 B2RF,” “Fibermax 960 B2R,” “Stoneville 5242 BR,” and “Stoneville 5599 BR.” Tolerance indices (TI) among these cultivars varied little, with “Deltapine 20B”, “DES 119”, and “DynaGrow 2520 B2RF” having the highest TI values (Table 7).
tSeed cotton yield taken from the middle two rows of subplots. |
uAldicarb applied in furrow at planting (0.84 kg a.i./ha) plus side dress application at pinhead square (1.17 kg a.i./ha).
vTI = tolerance index = [(seed cotton yield in nontreated plot)/(seed cotton yield in nematicide-treated plot)]*100; values listed only for cultivars meeting tolerance criteria.
wFor each cultivar, mean seed cotton yields followed by the same letter are not significantly different from each other at the 0.05 level based on ANOVA.
xMeans followed by the same letter in the TI column are not significantly different at the 0.10 level based on differences of least squares means.
yControl cultivar with respect to yield.
“Croplan Genetics 3520 B2RF,” “Deltapine 20B,” “Deltapine 488 BG/RR,” “DES 119” (tolerant control), “DynaGrow 2520 B2RF,” “Fibermax 960 B2R,” “Stoneville 5242 BR,” and “Stoneville 5599 BR” were identified as tolerant to reniform nematode in this study. Because yields were not significantly reduced in reniform nematode infested soils, any of these cultivars should be considered a suitable candidate for use in infested fields. However, yield potential must also be considered when selecting a cultivar for production. Of the tolerant cultivars identified, “Croplan Genetics 3520 B2RF” and “Stoneville 5242 BR” consistently ranked among the highest yielding entries in the study. Cotton growers in the Mississippi Delta farming on reniform nematode infested soils could realize immediate benefits from using identified tolerant cultivars. Growers outside this region should consult their local variety test extension publication and use those results in combination with reports of tolerance to make the best cultivar choice possible for their farm. The utility of tolerant cultivars that did not rank among the top yielding cultivars, such as “DES 119” and “Fibermax 960 B2R,” should not be dismissed from consideration. In soils were a high number of reniform nematodes is the most significant yield limiting factor, growers may still benefit from a tolerant, albeit lower yielding cultivar.
In this study, six of the 12 cultivars evaluated demonstrated levels of tolerance to reniform nematode, along with the tolerant control “DES 119.” More tolerant cultivars were identified in the present study than what has previously been reported in similar studies (2 of 34 entries reported by Usery et al. , and 3 of 39 entries reported by Stetina et al. ), but the reason for this is unclear. The presence of a significant nematicide x field interaction suggests that environment plays an important role in the ability to detect tolerance. Cotton breeders may have intentionally selected for tolerance to reniform nematode in recent years as they developed lines adapted to the Midsouth. Because tolerant lines do suffer the same yield loss as their susceptible counterparts, perhaps indirect selection for tolerance has occurred during the development of new, high yielding cultivars in a region where selection pressure from reniform nematode is common.
Previous studies have relied upon a single RI over the course of the growing season [15, 16] to identify host tolerance to nematodes. However, using two RI over the course of the growing season allows for verification of nematicide efficacy in addition to characterizing reniform nematode reproduction [13, 20, 21, 39]. Differences between nematicide-treated and -nontreated plots for early season RI indicated that reniform nematode reproduction was suppressed in the presence of aldicarb in this study. However, it should be noted that aldicarb is used for control of thrips, aphids, fleahoppers, leafminers, mites, overwintering boll weevil, plant bugs, and whiteflies, as well as nematodes . Because of this, alternative insecticides were applied across the field as needed to provide protection to plots that were not treated with aldicarb. Reddy et al.  showed aldicarb has a direct regulatory effect on plant growth when pests are absent. The addition of aldicarb also changes root distribution, with deeper root systems under ideal water and nutrient environment . These additional aldicarb properties may have influenced seed cotton yield in this study to favor aldicarb treated plots in some instances.
Until cultivars with resistance to reniform nematode are commercially available, tolerant cultivars could serve to reduce economic losses associated with cotton production in reniform nematode infested fields. As such, there is a need to continue programs directed at identifying current cultivars and advanced breeding lines tolerant to reniform nematode.
The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture (USDA) or Mississippi State University.
This research was partially funded by USDA, Agricultural Research Service Project no. 6402–22000-005-06S. USDA is an equal opportunity provider and employer.
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Copyright © 2012 Julie A. Blessitt et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.