Transgene Pyramiding of the HVA1 and mtlD in T3 Maize (Zea mays L.) Plants Confers Drought and Salt Tolerance, along with an Increase in Crop Biomass
Figure 3
(a)PCR analysis of the combination of HVA1-mtlD in T1 maize lines. (b) Northern blot hybridization of T1 HVA1 in HVA1-mtlD transgenic lines using the HVA1 probe. (c) Northern blot hybridization of T1 mtlD in the HVA1-mtlD transgenic lines using the mtlD probe. (d) Northern blot hybridization of T2 HVA1 in HVA1-mtlD transgenic lines using the HVA1 probe. (e) RCR analysis of the T3 HVA1-mtlD confirming the stability of both transgenes in the fourth (T3) progeny obtained from northern blot positive HVA1 and mtlD T2 line number 51-1.
