Efficient Shoot Organogenesis Using Leaf Disc and Nodal Explants of Passion Fruit (Passiflora edulis Sims) and Genetic Fidelity Assessment Using Sequence-Related Amplified Polymorphism (SRAP) Markers

Figure 3

SRAP amplification profiles of mother plants and randomly selected in vitro regenerated of passion fruit of KPF 4 variety. Lane L is a 1 kb plus molecular weight DNA marker (Generuler, Thermo scientific); Lanes, SK1 and SK2, are plantlets micropropagated on MS + 1 mg·L⁻¹ KIN; SK6 is a plantlet micropropagated on MS + 2 mg·L⁻¹ KIN; SK11 and SK12 are plantlets micropropagated on MS + 3 mg·L⁻¹ KIN; SB6 represents a mother plant; SB1 is a plantlet micropropagated on MS + 1 mg·L⁻¹ BAP; SB7 is a plantlet micropropagated on MS + 2 mg·L⁻¹ BAP; SB11 and SB12 are plantlets regenerated on MS + 3 mg·L⁻¹ BAP; SBK1, SBK 2, and SBK 3 are plantlets micropropagated on MS supplemented with 2 mg·L⁻¹ BAP + 0.5 mg·L⁻¹ KIN; SBK 4 represents a mother plant.