Research Article
Evaluation of Fructooligosaccharides and Inulins as Potentially Health Benefiting Food Ingredients by HPAEC-PED and MALDI-TOF MS
Table 1
Gradient elution program to elute
(method 1) FOS and inulin and (method 2) scFOS.
| HPAEC-PED method 1 | HPAEC-PED method 2 | Elution time (min) | A(%) | B(%) | (%) | Comment | Elution time (min) | A(%) | B(%) | (%) | Comment |
| | 0 | 100 | 0 | Start cleaning step | | 0 | 100 | 0 | Start cleaning step | | 0 | 100 | 0 | End cleaning step | | 0 | 100 | 0 | End cleaning step | | 89 | 10 | 1 | Start conditioning step | | 89 | 10 | 1 | Start conditioning step | 0 | 89 | 10 | 1 | End conditioning step | 0 | 89 | 10 | 1 | End conditioning step | 0.1 | 89 | 10 | 1 | Injection, acquisition start | 0.1 | 89 | 10 | 1 | Injection, acquisition start | 4 |
84 | 15 |
1 | End first gradient step | 5 | 89 | 10 | 1 | End isocratic elution | 45 | 60 | 20 | 20 | End first gradient step | 80 | 79 | 15 | 40 | End third gradient step | 55 | 50 | 20 | 30 | End second gradient step |
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A: deionized water; B: sodium hydroxide (600 mM); : sodium
nitrate (250 mM); : sodium
acetate (500 mM). time indicates time prior to sample
injection.
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