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International Journal of Analytical Chemistry
Volume 2009, Article ID 596379, 12 pages
http://dx.doi.org/10.1155/2009/596379
Research Article

Kinetic Spectrophotometric Determination of Certain Cephalosporins in Pharmaceutical Formulations

Analytical Chemistry Department, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt

Received 4 August 2008; Accepted 16 March 2009

Academic Editor: Manuel P. Soriaga

Copyright © 2009 Mahmoud A. Omar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple, reliable, and sensitive kinetic spectrophotometric method was developed for determination of eight cephalosporin antibiotics, namely, Cefotaxime sodium, Cephapirin sodium, Cephradine dihydrate, Cephalexin monohydrate, Ceftazidime pentahydrate, Cefazoline sodium, Ceftriaxone sodium, and Cefuroxime sodium. The method depends on oxidation of each of studied drugs with alkaline potassium permanganate. The reaction is followed spectrophotometrically by measuring the rate of change of absorbance at 610 nm. The initial rate and fixed time (at 3 minutes) methods are utilized for construction of calibration graphs to determine the concentration of the studied drugs. The calibration graphs are linear in the concentration ranges 5–15  𝜇 g  m L − 1 and 5–25  𝜇 g  m L − 1 using the initial rate and fixed time methods, respectively. The results are validated statistically and checked through recovery studies. The method has been successfully applied for the determination of the studied cephalosporins in commercial dosage forms. Statistical comparisons of the results with the reference methods show the excellent agreement and indicate no significant difference in accuracy and precision.