Selective Spectrophotometric and Spectrofluorometric Methods for the Determination of Amantadine Hydrochloride in Capsules and Plasma via Derivatization with 1,2-Naphthoquinone-4-sulphonate

Table 1

Effect of diluting and extracting solvents on the intensity of the reaction product of AMD with NQS (0.06%, w/v). Values for all solvents are mean of three determinations; the RSDs for the readings were <3.

Spectrophotometric method		Spectrofluorometric method
Extracting solvent	Absorbance	Diluting solvent	Fluorescence intensity
Carbon tetrachloride	0.487	Methanol	65.66
Chloroform	0.718	Ethanol	78.38
Dichloromethane	0.616	Isopropanol	63.02
Ethyl-acetate	0.362	Acetone	2.00
Toluene	0.267	Acetonitrile	16.18
Benzene	0.239	Dimethylformamide	3.94
		1,4-Dioxane	76.11