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International Journal of Analytical Chemistry
Volume 2010, Article ID 419439, 8 pages
http://dx.doi.org/10.1155/2010/419439
Research Article

Electrochemical Performances of Electroactive Nano-LayeredOrganic-Inorganic Perovskite Containing Trivalent Iron Ion and its Use for a DNA Biosensor Preparation

1State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China
2State-key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan university, Changsha, Hunan 410082, China
3School of Materials Science Engineering, Wuhan Institute of Technology, Wuhan, Hubei 430073, China

Received 2 September 2009; Revised 11 March 2010; Accepted 18 March 2010

Academic Editor: Shi-Gang Sun

Copyright © 2010 Jing Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A steady nano organic-inorganic perovskite hybrid with Fe(CN)6 (3-AMP = 3-methylaminopyridine) was prepared in the air. The structure is an unusual layered organic-inorganic type. The resulting hybrid enveloped in paraffin to prepare Fe(CN)6 paste electrode (HPE) shows good electrochemical activity and a couple of oxidation and reduction peaks with potential of cyclic voltammometry (CV) at around 440 mV and 30 mV. Compared with that on CPE, oxidation potential of on HPE shifts negatively 259.7 mV and that of reduction shifts positively 338.7 mV, which exhibits that Fe(CN)6 can accelerate the electron-transfer to improve the electrochemical reaction reversibility. Such characteristics of Fe(CN)6 have been employed to prepare the DNA biosensor. The single-strand DNA (ssDNA) and double-strand DNA (dsDNA) immobilized on HPE, respectively, can improve the square wave voltammometry (SWV) current and SWV potential shifts positively. The effect of pH was evaluated. And there is hybridization peak on SWV curve using HPE immobilized ssDNA in the complementary ssDNA solution. And HPE immobilized ssDNA can be utilized to monitor the DNA hybridization and detect complementary ssDNA, covering range from to  g/mL with detection limit of  g/mL. The DNA biosensor exhibits a good stability and reproducibility.