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International Journal of Analytical Chemistry
Volume 2010, Article ID 478143, 5 pages
Research Article

A Validated RP HPLC-PAD Method for the Determination of Hederacoside C in Ivy-Thyme Cough Syrup

1Faculty of Pharmacy, Applied Sciences University, Amman 11931, Jordan
2Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan (UJ), Amman 11942, Jordan
3Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Jordan (UJ), Amman 11942, Jordan
4Department of Clinical Pharmacy and Biopharmaceutics, Faculty of Pharmacy, University of Jordan (UJ), Amman 11942, Jordan
5Sana Pharmaceutical Research Co., Amman 11931, Jordan

Received 22 March 2010; Revised 20 June 2010; Accepted 2 July 2010

Academic Editor: Yijun Chen

Copyright © 2010 Ayman Khdair et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A simple reversed phase high-performance liquid chromatographic (RP-HPLC) method coupled with a photodiode array detector (PAD) has been developed and validated for the analysis of hederacoside C, the marker of ivy plant, in Ivy-Thyme cough syrup. Separation of hederacoside C was achieved using a Phenomenex-Gemini C18 column isothermally at 4 0 C. A mobile phase system constituted of solvent A (water: acetonitrile: orthophosphoric acid (85%), 860 : 140 : 2 v/v) and solvent B (acetonitrile: orthophosphoric acid (85%), 998 : 2 v/v) was used, at gradient conditions, at a flow rate of 1.5 mL/min. Analysis was performed using UV-detection (205 nm). The method was linear over the range (0.03–0.15) mg/mL of hederacoside C ( 𝑟 = 0 . 9 9 9 2 ). Repeatability and intermediate precision were acceptable (RSD < 2 %). Limits of detection (LOD) and quantitation (LOQ) were 0.011 and 0.032 mg/mL, respectively. Percentage recovery was found to lie between 99.69% and 100.90% (RSD < 2 %). The method was also proved to be specific (peak-purity c o e c i e n t = 0 . 9 9 6 ).