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International Journal of Analytical Chemistry
Volume 2011, Article ID 832414, 6 pages
http://dx.doi.org/10.1155/2011/832414
Research Article

Quantification of Lansoprazole in Oral Suspension by Ultra-High-Performance Liquid Chromatography Hybrid Ion-Trap Time-of-Flight Mass Spectrometry

1Department of Pharmaceutical Sciences, Bill Gatton College of Pharmacy East Tennessee State University, Box 70594, Johnson City, TN 37614, USA
2Department of Pharmacy Practice, Bill Gatton College of Pharmacy East Tennessee State University, Box 70594, Johnson City, TN 37614, USA

Received 11 January 2011; Revised 7 April 2011; Accepted 13 April 2011

Academic Editor: Hian Kee Lee

Copyright © 2011 Stacy D. Brown et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An LC-MS/MS method was developed and validated to be used as a stability indicating assay for the study of a 3 mg/mL lansoprazole oral suspension. The method utilizes a UPLC (ultra-performance liquid chromatography) column and unique mass spectrometric detection (ion-trap time-of-flight (IT-TOF)) to achieve a sensitive (LOD 2 ng/mL), accurate, and reproducible quantification of lansoprazole. This method reports an intraday and interday coefficient of variation of 2.98 ± 2.17% (n=5 for each concentration for each day) and 3.07 ± 0.89% (n=20 for each concentration), respectively. Calibration curves (5–25 μg/mL) were found to be linear with an R2 value ranging from 0.9972 to 0.9991 on 4 different days. Accuracy of the assay, expressed as % error, ranged from 0.30 to 5.22%. This method is useful for monitoring the stability of lansoprazole in oral suspension.