Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract
Eastern blotting of ginsenosides in traditional Chinese medicine formulas using anti-ginsenoside Rb1 MAb. Samples (1) Jigengtang, (2) Dahuanggancaotang, (3) Nenshenyangyongtang, (4) Sijunzitang, (5) Nenshentang, (6) Buanxiaxiexintang, (7) Xiaocaihutang, and (8) Crude ginseng extract. Lanes 1 and 2 do not contain ginseng. Standard of ginsenosides indicated, ginsenosidr-Rg1, -Re, -Rd, -Rc, and -Rb1, respectively, from the upper spot.
(b) Eastern blotting
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