Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract
Eastern blotting profile of glycyrrhizin in rat serum after injection of glycyrrhizin (a) TLC of rat-serum-injected glycyrrhizin TLC was developed with n-BuOH-H2O-AcOH (7 : 2 : 1). Spots were detected by 10% H2SO4. (b) Eastern blotting of rat-serum-injected glycyrrhizin; the band of glycyrrhizin was detected until 1 h after injection. GA: glycyhrrhetinic acid, GC: glycyrrhizin, and GG: glycyrrhetinic acid monoglucuronide.
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