Figure 8: Pharmacologic effects on nanosensors at 37°C. Cells incubated at 37°C with the addition of bafilomycin or amiloride for two hours, followed by the addition of nanosensors. (a) Demonstration of total pixel/nanosensor number averaged from three individual cells. Cells are incubated at 37°C untreated or treated for two hours with drug, followed by the addition of nanosensors for ten minutes, then imaged. (b) Demonstration of the percentage of nanosensors averaged from three cells, from the whole, within the assigned pH groupings. Reprinted with permission from Nowak-Lovato et al. [11] (copyright 2010 Springer).