Research Article
Two Validated Spectrofluorometric Methods for Determination of Gemifloxacin Mesylate in Tablets and Human Plasma
Table 3
Robustness of the proposed methods.
| Method | Experimental parameter variation | Recovery (%) SDa |
| | No variationb | | | SDS volume (L) | | | 90 | | | 110 | | | pH | | Micelle-enhanced spectrofluorometric | 5.3 | | | 5.7 | | | Temperature (°C) | | | 20 | | | 30 | |
| | No variationb | | | pH | | | 3.3 | | | 3.7 | | | Acetylacetone volume (mL) | | | 0.4 | | | 0.6 | | Hantzsch reaction | Formaldehyde volume (mL) | | | 0.8 | | | 1.2 | | | Temperature (°C) | | | 95 | | | 105 | | | Heating time (min) | | | 18 | | | 22 | |
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Mean of three determinations. Following the general assay procedure conditions.
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