Isolation, Characterization, and RP-HPLC Estimation of P-Coumaric Acid from Methanolic Extract of Durva Grass (Cynodon dactylon Linn.) (Pers.)

Table 3

Optimised chromatographic conditions, system suitability, and recovery study.

Parameter	Chromatographic conditions
Instrument	SHIMADZU LC-20AT Prominence liquid chromatograph
Column	WELCHROM C18 column (250 mm × 4.6, 5 µm)
Detector	SHIMADZU SPD-20A Prominence UV-Vis detector
Diluents	Mobile phase
Mobile phase	Water : methanol : glacial acetic acid (65 : 34 : 1 v/v)
Flow rate	1 mL/min
Detection wave length	UV at 310 nm
Run time	15 minutes
Column back pressure	114 kgf
Temperature	Ambient temperature (25°C)
Injection volume	20 µL
System suitability parameters
Retention time (min.)	6.617
Theoretical plates [th.pl] (Efficiency)	7881
Tailing factor (asymmetry)	1.209
Recovery study of P-coumaric acid
Serial number	1	2	3
Level of spiking of standard	50%	100%	125%
Amount of reference standard added to preanalysed samples (g/mL)	2.4	4.8	6.0
Amount found^*; (g/mL)	7.19	9.58	10.77
Percentage of; recovery^*  ±  S.D ()	99.86	99.80	99.72

Mean of three determinations.