Development of HPLC Protocol and Simultaneous Quantification of Four Free Flavonoids from Dracocephalum heterophyllum Benth.

Table 2

Data obtained from the five replicate HPLC analyses for accuracy and reproducibility.

Number of replicates	Luteolin		Kaempferol		Diosmetin		Chrysosplenetin
	Rt	Area	Rt	Area	Rt	Area	Rt	Area
1	38.85	34.58	41.58	38.90	42.34	56.63	45.78	158.62
2	38.88	35.08	41.62	39.48	42.37	57.93	45.80	162.15
3	38.85	35.64	41.58	40.02	42.34	59.09	45.80	165.60
4	38.87	36.29	41.60	40.62	42.35	60.36	45.78	169.33
5	38.90	36.20	41.64	38.75	42.38	56.46	45.83	166.19
Total	194.35	177.79	208.02	197.77	211.78	290.47	228.99	821.89
Average	38.87	35.55	41.60	39.55	42.35	58.09	45.79	164.37
SD	0.02	0.73	0.02	0.77	0.01	1.65	0.02	4.10
RSD%	0.05	2.05	0.06	1.97	0.04	2.85	0.04	2.49

Rt: retention time.