Development and Validation of Simple RP-HPLC Method for Intracellular Determination of Fluconazole Concentration and Its Application to the Study of Candida albicans Azole Resistance

Figure 3

Typical chromatogram of fungi biomass blank with IS tinidazole (a). UV spectra of peak with RT at approximately 13 min (b) and UV spectra of peak with RT at approximately 15 min (c).

(a)

(b)

(c)