Stability-Indicating Assay for the Determination of Pentobarbital Sodium in Liquid Formulations

Table 2

Forced degradations studies.

Stress conditions	% remaining	% degradation	Retention time of degraded products
Acidic stress (1 N HCl, 50°C, 1 h)	102.2	—	0.0
High acidic stress (12 N HCl, 50°C, 48 h)	31.8	68.2	0.0
Alkaline stress (1 N NaOH, 50°C, 1 h)	98.4	1.6	0.0
High alkaline stress (10 N NaOH, 50°C, 48 h)	89.8	10.2	1.46, 1.81
Oxidative stress (3%, 50°C, 48 h)	50.9	49.1	1.44
Thermal stress (50°C, 5 days)	89.4	10.6	0.0
High thermal stress (100°C, 1 h)	19.9	80.1	0.0
Direct sunlight (48 h)	95.1	4.9	0.0
Aqueous stability (after 21 days)	99.7	0.3	0.0