Stability-Indicating Assay for the Determination of Pentobarbital Sodium in Liquid Formulations

Table 3

Linearity data of the developed method.

Initial conc. (g/mL)	Mean peak area ± S.D.; (pentobarbital) ()	Mean peak area (IS)	Mean peak ratio	Actual conc. (g/mL)	% assay
5	4.285 ± 0.020	27.805 ± 0.192	0.154 ± 0.001	3.99 ± 1.29	79.8
10	8.637 ± 0.050	27.897 ± 0.284	0.310 ± 0.001	9.33 ± 1.29	93.3
20	17.098 ± 0.143	27.186 ± 1.093	0.630 ± 0.031	20.34 ± 1.19	101.7
50	42.565 ± 1.147	27.983 ± 0.376	1.521 ± 0.028	50.96 ± 1.20	101.9
100	83.084 ± 1.149	27.856 ± 0.111	2.983 ± 0.047	101.19 ± 1.14	101.2
200	164.194 ± 0.401	28.135 ± 0.253	5.836 ± 0.048	199.26 ± 1.13	99.6
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