Table of Contents Author Guidelines Submit a Manuscript
International Journal of Analytical Chemistry
Volume 2015, Article ID 748056, 5 pages
http://dx.doi.org/10.1155/2015/748056
Research Article

Development of a Rapid and Simple Method for Detection of Protein Contaminants in Carmine

1Graduate School of Environmental Symbiotic System and Department of Innovative Systems Engineering, Nippon Institute of Technology, 4-1 Gakuendai, Miyashiro-machi, Saitama 345-8501, Japan
2Kishi Kasei Company, 1-10-8 Fukuura, Kanazawa-ku, Yokohama 234-0004, Japan

Received 1 December 2014; Revised 16 March 2015; Accepted 19 March 2015

Academic Editor: Shaoping Li

Copyright © 2015 Norihisa Nakayama et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. W. Dapson, “The history, chemistry and modes of action of carmine and related dyes,” Biotechnic and Histochemistry, vol. 82, no. 4-5, pp. 173–187, 2007. View at Publisher · View at Google Scholar · View at Scopus
  2. Food and Drug Administration, H. 73,100 Cochineal Extract, Carmine, 21 CFR Ch. 73,100, 4-I-04 edition, 2014.
  3. The European Parliament and the Councile of the EU, “The European Parliament and the Councile Directive 94/36/EC of 30 June 1994 on coluours for use in foodstuffs,” Official Journal of the European Communities, no. L237/13-29, 10.9.94, 1994. View at Google Scholar
  4. Japanese Consumer Affairs Agency, Attention Awakening about the Cochineal Pigment, 2012.
  5. B. Añíbarro, J. Seoane, C. Vila, V. Múgica, and M. Lombardero, “Occupational asthma induced by inhaled carmine among butchers,” International Journal of Occupational Medicine and Environmental Health, vol. 16, no. 2, pp. 133–137, 2003. View at Google Scholar · View at Scopus
  6. M. T. Lizaso, I. Moneo, B. E. García, S. Acero, S. Quirce, and A. I. Tabar, “Identification of allergens involved in occupational asthma due to carmine dye,” Annals of Allergy, Asthma and Immunology, vol. 84, no. 5, pp. 549–552, 2000. View at Publisher · View at Google Scholar · View at Scopus
  7. Y. Ohgiya, F. Arakawa, H. Akiyama et al., “Molecular cloning, expression, and characterization of a major 38-kd cochineal allergen,” Journal of Allergy and Clinical Immunology, vol. 123, no. 5, pp. 1157–1162, 2009. View at Publisher · View at Google Scholar · View at Scopus
  8. T. Ichi, T. Koda, C. Yukawa, M. Sakata, and H. Sato, “Purified cochineal pigment and process for producing the same,” WO/2002/022743, 2002. View at Google Scholar
  9. A. L. Shapiro, E. Viñuela, and J. V. Maizel Jr., “Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels,” Biochemical and Biophysical Research Communications, vol. 28, no. 5, pp. 815–820, 1967. View at Publisher · View at Google Scholar · View at Scopus
  10. R. C. Switzer III, C. R. Merril, and S. Shifrin, “A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels,” Analytical Biochemistry, vol. 98, no. 1, pp. 231–237, 1979. View at Publisher · View at Google Scholar · View at Scopus