Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation

Figure 1

CBB stained gel from the 1D SDS-PAGE of proteins extracted from liver tumor samples using various extraction buffers. The loading concentration of each sample reflects the amount of proteins extracted from the liver samples before trypsin digestion. Larger number of protein bands would mean larger number of proteins extracted. Darker protein bands from each lane would mean a higher amount of proteins extracted. Black boxes indicate the two best extraction buffers and conditions in terms of number of protein bands and the intensity of the CBB stain. The highlighted regions for Lanes 2 and 7 show a larger number of visible bands compared to other lanes. Δ: heat.