Sample Preparation and Extraction in Small Sample Volumes Suitable for Pediatric Clinical Studies: Challenges, Advances, and Experiences of a Bioanalytical HPLC-MS/MS Method Validation Using Enalapril and Enalaprilat
Table 2
Relative matrix effect obtained in seven different human sources.
Donor
Enalapril
Enalaprilat
0.39 ng/mL
200 ng/mL
0.35 ng/mL
180 ng/mL
Healthy adults
29–86 years old
♂ Donor 1
106.7
96.8
162.5
135.9
♂ Donor 2
99.1
95.7
122.1
108.2
♂ Donor 3
96.6
96.6
120.9
119.4
♀ Donor 4
111.1
97.6
132.1
126.6
♀ Donor 5
107.2
97.8
121.0
114.5
♀ Donor 6
98.8
94.5
123.1
112.1
♂ Donor 7
98.4
92.8
114.4
106.7
Mean value of normalized relative ME ± S.D. [%]
102.6 ± 5.6
96.0 ± 1.8
128.0 ± 16.1
117.6 ± 10.5
CV [%] within donors
5.49
1.87
12.56
8.96
ME: matrix effect; S.D.: standard deviation; CV: coefficient of variation.
