Table of Contents Author Guidelines Submit a Manuscript
International Journal of Analytical Chemistry
Volume 2016, Article ID 1029286, 8 pages
http://dx.doi.org/10.1155/2016/1029286
Research Article

Multianalytical Method Validation for Qualitative and Quantitative Analysis of Solvents of Abuse in Oral Fluid by HS-GC/MS

1Postgraduate Program in Pharmaceutical Sciences, School of Pharmacy, Federal University of Rio Grande do Sul, Avenida Ipiranga 2752, Santana, 90610-000 Porto Alegre, RS, Brazil
2Department of Statistics, Federal University of Rio Grande do Sul, Avenida Bento Gonçalves 9500, 91509-200 Porto Alegre, RS, Brazil
3Center for Drug and Alcohol Research, Federal University of Rio Grande do Sul, Rua Ramiro Barcelos 2350, Santana, 90035-903 Porto Alegre, RS, Brazil

Received 8 December 2015; Revised 20 March 2016; Accepted 6 April 2016

Academic Editor: Dimitrios Tsikas

Copyright © 2016 Bruna Claudia Coppe et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The use of oral fluid as a biological matrix to monitor the use of drugs of abuse is a global trend because it presents several advantages and good correlation to the blood level. Thus, the present work aimed to develop and validate an analytical method for quantification and detection of solvents used as inhalants of abuse in oral fluid (OF), using Quantisal as collector device by headspace and gas chromatography coupled with a mass detector (HS-GC/MS). Chromatographic separation was performed with a ZB-BAC1 column and the total time of analysis was 11.8 min. The method showed good linearity (correlation coefficient higher than 0.99 for all solvents). The limits of detection ranged from 0.05 to 5 mg/L, while the lower limits of quantification ranged from 2.5 to 12.5 mg/L. Accuracy, precision, matrix effect, and residual effect presented satisfactory results, meeting the criteria accepted for the validation of bioanalytical methods. The method showed good selectivity considering that, for solvents coeluting at the same retention time, resolution was performed by the mass detector. The method developed proved to be adequate when applied in OF samples from users of drugs and may be used to monitor the abuse of inhalants in routine forensic analyses.