Research Article

Enantioseparation of Citalopram by RP-HPLC, Using Sulfobutyl Ether-β-Cyclodextrin as a Chiral Mobile Phase Additive

Figure 5

Typical chromatogram of CIT enantiomers. Mobile phase: aqueous buffer/methanol/acetonitrile of 21 : 3 : 1 (v/v/v), 5 mM sodium dihydrogen phosphate, 12 mM SBE-β-CD, pH of 2.5, flow rate of 1.0 mL/min, detection wavelength of 240 nm, temperature of 25°C, and injection of 10 μL and 0.22 mM of racemic CIT.