Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks
Table 5
Validation of the simultaneous determination of food additives and caffeine by developed method potassium sorbate (SOR), sodium benzoate (BEN), carmoisine (CAR), allura red (ALU), ponceau 4R (PON), and caffeine (CAF).
Levels
SOR (μg/mL)
BEN (μg/mL)
CAR (μg/mL)
ALU (μg/mL)
PON (μg/mL)
CAF (μg/mL)
10.0
15.0
20.0
10.0
15.0
20.0
4.0
6.0
8.0
4.0
6.0
8.0
4.0
6.0
8.0
10.0
15.0
20.0
Intra-assay
9.97
15.22
19.76
9.70
15.37
20.18
4.05
6.17
7.92
3.90
6.02
7.86
3.79
5.72
7.75
9.96
15.15
20.28
9.80
15.30
20.01
10.02
15.09
20.21
4.15
6.33
8.01
3.83
5.97
7.82
3.89
5.92
7.91
9.99
15.50
20.02
9.91
15.31
19.86
10.03
14.79
20.38
4.18
6.20
8.11
3.87
6.06
7.70
3.92
5.87
8.04
10.03
15.16
20.20
Mean
9.89
15.28
19.88
9.92
15.08
20.26
4.13
6.23
8.01
3.87
6.02
7.79
3.87
5.84
7.91
9.99
15.27
20.16
RSD%
0.869
0.321
0.634
1.895
1.923
0.533
1.646
1.364
1.186
0.904
0.747
1.060
1.757
1.781
1.643
0.350
0.310
0.689
Recovery%
98.90
101.87
99.40
99.20
100.53
101.30
103.25
103.83
100.13
96.75
100.33
97.83
96.75
97.33
98.88
99.90
101.80
100.80
Interassay
9.92
15.09
19.66
9.84
14.76
20.71
4.14
6.01
7.81
3.91
6.01
7.96
4.10
6.01
8.20
10.35
15.10
20.09
9.84
15.23
19.59
9.85
14.95
20.74
4.18
6.06
8.14
3.85
5.84
7.90
4.08
6.18
8.26
10.01
15.32
20.43
9.77
15.21
19.69
9.81
14.79
20.25
4.05
6.19
7.96
3.81
5.86
8.04
3.83
6.20
8.24
10.17
14.88
20.07
9.68
15.40
19.89
10.02
14.82
20.11
4.19
6.22
7.98
3.76
6.08
7.82
4.01
5.98
7.99
10.03
15.16
20.20
Mean
9.80
15.23
19.71
9.88
14.83
20.45
4.14
6.12
7.97
3.83
5.95
7.93
4.03
6.09
8.14
10.14
15.11
20.20
RSD%
1.041
0.840
0.624
0.961
0.566
1.565
1.546
1.650
1.694
1.644
1.950
1.173
1.919
1.855
1.518
1.548
1.204
0.817
Recovery%
98.00
101.53
98.55
98.80
98.87
102.25
103.50
102.00
99.62
95.75
99.17
99.13
100.75
101.50
102.12
101.40
100.73
101.00
Relative standard deviation, RSD (%), standard deviation/mean × 100.