Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks

Table 6

Results of accuracy studies (mean value ± standard deviation, ).

Sample	Food additive	Sample concentration (g/mL)	Added (g/mL)	Found (g/mL)	Recovery (%)
Energy drink	Potassium sorbate	10.68	3	13.70 ± 0.048	100.67
			6	16.90 ± 0.057	103.67
			9	20.00 ± 0.065	103.56
	Caffeine	7.11	3	10.18 ± 0.045	102.33
			6	13.25 ± 0.054	102.33
			9	16.61 ± 0.042	105.56
	Allura red	3.26	1	4.31 ± 0.038	105.00
			2	5.34 ± 0.037	104.00
			3	6.39 ± 0.044	104.33
Pomegranate juice	Potassium sorbate	12.71	3	15.64 ± 0.059	97.67
			6	18.61 ± 0.103	98.33
			9	21.74 ± 0.166	100.34
	Sodium benzoate	7.24	3	10.34 ± 0.040	103.60
			6	13.30 ± 0.054	101.20
			9	16.45 ± 0.063	102.50
	Carmoisine	1.21	1	2.25 ± 0.012	104.00
			2	3.30 ± 0.017	104.50
			3	4.34 ± 0.022	104.40
	Ponceau 4R	4.08	1	5.11 ± 0.036	102.66
			2	6.16 ± 0.039	104.16
			3	7.15 ± 0.045	102.22