Selective Sensing of Tyrosine Phosphorylation in Peptides Using Terbium(III) Complexes

Figure 10

(a) -Lc1yne and (b) its conjugate with Abltide peptide prepared by click reaction. In (c), phosphorylation of Abltide by tyrosine kinases Abl was monitored in real-time using the conjugate by measuring time-resolved luminescence at 545 nm. The concentrations of Abl were 1000 (red), 100 (blue), and 10 ng/mL (green). Conditions: [the conjugate] = 5 μM, [ATP] = 100 μM, [MgCl₂] = 1 mM, and [NaCl] = 7.5 mM. Reproduced with permission from [38]. Copyright 2015, American Chemical Society.

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