The Development of DNA Based Methods for the Reliable and Efficient Identification of Nicotiana tabacum in Tobacco and Its Derived Products

Figure 4

Practical sample detection with different practical samples. (a) LAMP method through direct visual detection with SYBR Green I; (b) LAMP method on 2% agarose gel electrophoresis analysis; (c) qPCR method. Lane 1: NTC; lane 2: PTC; lanes 3-4: coriander and spinach; lanes 5-6: burley and Oriental SAADI-6; lanes 7-8: NT. Oriental and NT. Virginia gold; lanes 9-10: reconstituted tobacco (no tobacco stems, no sulphate cellulose) and reconstituted tobacco (tobacco stems, with sulphate cellulose); lanes 11-12: cigarette (Liqun and Double Happiness); lanes 13-14: wrappers from Liqun and Marlboro, respectively; lane M: Trans 2K DNA marker. Ct was expressed as mean Ct SD from 3 independent experiments with three replications.

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