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International Journal of Analytical Chemistry
Volume 2017 (2017), Article ID 1389807, 6 pages
Research Article

Simple Quantification of Pentosidine in Human Urine and Plasma by High-Performance Liquid Chromatography

1College of Pharmacy and Research, Institute of Pharmaceutical Science and Technology, Ajou University, Suwon 16499, Republic of Korea
2Department of Endocrinology and Metabolism, Ajou University School of Medicine, Suwon 16499, Republic of Korea

Correspondence should be addressed to So Hee Kim

Received 13 June 2017; Accepted 13 September 2017; Published 18 October 2017

Academic Editor: Mohamed Abdel-Rehim

Copyright © 2017 Ji Sang Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Pentosidine is an advanced glycation end-product (AGE) and fluorescent cross-link compound. A simple high-performance liquid chromatographic (HPLC) method was developed for the detection and quantification of pentosidine in human urine and plasma. The mobile phase used a gradient system to improve separation of pentosidine from endogenous peaks, and chromatograms were monitored by fluorescent detector set at excitation and emission wavelengths of 328 and 378 nm, respectively. The retention time for pentosidine was 24.3 min and the lower limits of quantification (LLOQ) in human urine and plasma were 1 nM. The intraday assay precisions (coefficients of variation) were generally low and found to be in the range of 5.19–7.49% and 4.96–8.78% for human urine and plasma, respectively. The corresponding values of the interday assay precisions were 9.45% and 4.27%. Accuracies (relative errors) ranged from 87.9% to 115%. Pentosidine was stable in a range of pH solutions, human urine, and plasma. In summary, this HPLC method can be applied in future preclinical and clinical evaluation of pentosidine in the diabetic patients.