Simple Quantification of Pentosidine in Human Urine and Plasma by High-Performance Liquid Chromatography

Figure 2

Chromatograms after deproteinization of a human urine blank (0.663 nM) (a), urine standard spiked with 1 nM of pentosidine (b), and type 2 diabetes mellitus (T2DM) patient’s urine (2.28 nM) (c). The arrows show the pentosidine peak. The human urine used in (a) and (b) was obtained from a healthy male subject.

(a)

(b)

(c)