GC Method Validation for the Analysis of Menthol in Suppository Pharmaceutical Dosage Form

Table 1

The GC parameters.

Carrier gas	Helium
Column oven temperature (programmed)	Rate (°C/minute)	Temperature (°C)	Hold time (minute)	Total time (minute)
	Initial	90	1.0	1.0
	15	181	3.0	18.0
	Total Time			19.0
Detector temperature	180°C
Injector temperature	280°C
Flow rate	5.0 mL/ minute (constant)
Split ratio	50
Injection volume	1 µL
Gases flow rate	Makeup Gas
	He → 30 mL/minutes
	H2 → 30 mL/minutes
	Air → 300 mL/minutes