NMR Confirmation and HPLC Quantification of Javamide-I and Javamide-II in Green Coffee Extract Products Available in the Market

Figure 2

HPLC chromatograms of the extract prepared from the mixed green coffee sample. The mixture of seven green coffee samples (1 Nature Made Inc., 2 BioRise Nutrition, 3 GMX Naturals, 4 Natrogix, 5 Pro Nutrient Labs, 6 The Health Life, and 7 Wellsome Nutrition) was extracted with 70% MeOH (50 mL). The extract was analyzed using the HPLC method as described in Materials and Methods, and the peaks of javamide-II (A) and javamide-I (B) were detected using an electrochemical detector and confirmed by NMR spectroscopic methods.