Research Article

Validation of a Liquid Chromatography-Tandem Mass Spectrometric Assay for Quantitative Analysis of Lenvatinib in Human Plasma

Table 1

Stability of lenvatinib in human plasma and stock solutions.

ConditionMatrixMean nominal conc.Mean measured conc.RecoveryCV
ng/mLng/mL%%

−70mLc.d1 month3.3 mM HCl/CH3OH578005530095.72.4
−2070HHC monthPlasma9.69.295.81.6
−208aHHC monthPlasma200.6201.3100.30.46
3 freeze and thaw cyclesPlasma11.510.490.41.6
3 freeze and thaw cyclesPlasma179.8155.686.50.79
Room temperature for 24 hoursPlasma11.511.398.22.6
Room temperature for 24 hoursPlasma179.8177.498.62.8
AutosamplerAfter treatment10.19.3927.3
AutosamplerAfter treatment199.3190.695.61.8