International Journal of Analytical Chemistry

Research Article

Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry

Table 1

Alkaloids characterized in F. capreolata by GC-QTOF-MS.


Peak N°	(min)	Proposed alkaloid	Precursor m/z (M⁺)	Molecular formula	MS score	Mass error (ppm)	EI-MS m/z (relative intensity, %)	Alkaloid type

1	17.5	Stylopine	323.1139	C₁₉H₁₇NO₄	94	3.96	323.1139 (44), 322.1071 (52), 148.0523 (100), 91.0541 (26)	Protoberberine

2	18.7	Protopine	353.1247	C₂₀H₁₉NO₅	94	3.53	163.0396 (19), 149.0564 (11), 148.0518 (100), 91.0539 (14)	Protopine

3	20.4	Cheilanthifoline	325.1296	C₁₉H₁₉NO₄	91	3.86	325.1289 (40), 324.1215 (46), 149.0581 (26), 148.0518 (100)	Protoberberine

4	21.2	Isoboldine	327.1438	C₁₉H₂₁NO₄	80	8.09	327.1438 (54), 326.1375 (100), 284.1048 (32), 253.0833 (27)	Aporphine

5	23.8	Coreximine	327.1428	C₁₉H₂₁NO₄	69	3.57	327.1436 (57), 326.1376 (63), 178.0841 (100), 176.069 (45)	Protoberberine

6	24.7	Dihydrosanguinarine	333.0971	C₂₀H₁₅NO₄	83	7.01	334.1014 (18), 333.0971 (88), 332.0899 (100), 105.0669 (15)	Benzophenanthridine

7	25.2	Fumariline	351.1089	C₂₀H₁₇NO₅	96	3.5	336.0862 (28), 323.1104 (25), 322.1061 (100), 264.1006 (29)	Spirobenzylisoquinoline

8	29.8	Parfumine	353.1246	C₂₀H₁₉NO₅	89	4.4	353.1246 (17), 338.1006 (29), 325.1252 (26), 324.1215 (100), 322.086 (31), 308.126 (31)	Spirobenzylisoquinoline

RT: retention time and EI-MS: electron impact-mass spectrometry.