Isocratic Resolution of Fluoroquinolone-Based Antibiotics on the Phenylethyl-Bonded Phase under Nonaqueous Elution: A Consideration of the Separation Mechanism

Figure 3

Resolution of 10 fluoroquinolone-based antibiotics and their precursors on the phenyl-bonded stationary phase under the elution of nonaqueous mobile phase of 490/10/1/2 by volume (acetonitrile/methanol/acetic acid/triethylamine, ) (a). The interaction simulation between ofloxacin and phenyl moiety expressed in stereochemistry molecular (b) and stick and ball (c) models and molecular structure (d) for easy comparison.

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