LC-MS/MS Quantification of Tramadol and Gabapentin Utilizing Solid Phase Extraction

Table 6

Stability results of TMD and GBP.

Stability experiment	Stability condition	%Mean stability
		TDL		FNS
		LQC	HQC	LQC	HQC
Auto sampler stability	41 h at 5°C	98.1	96.4	96.1	106.7
Free and thaw stability	5 cycles at -70°C ± 15°C	97.1	100.1	99.9	96.1
Dry extract stability	52 h at 2-8°C	94.8	96.8	101.2	96.6
Room temperature stability	16 h at room temperature at 25°C ± 5°C	94.1	102.8	91.7	97.2
Long term stability	32 days at -70°C ± 15°C	99.6	95.7	98.8	95.5
Stability in blood	2.5 h room temperature at 25°C ± 5°C	96.4	99.1	103.2	97.6