Rapid, Validated UPLC-MS/MS Method for Determination of Glibenclamide in Rat Plasma

Table 1

Representing gradient scheme of mobile phase.

Time in min	Mobile phase flow	Mobile Phase
		Component A	Component B
0.0	150 μl/min	85	15
0.70	150 μl/min	85	15
0.71	150 μl/min	100	0
0.80	150 μl/min	100	0
0.81	150 μl/min	85	15
2.0	150 μl/min	85	15