Rapid, Validated UPLC-MS/MS Method for Determination of Glibenclamide in Rat Plasma

Table 2

Results of validation studies.

Validation Parameter	Validation Results
	LLOQ	LOQ	MLOQ	HLOQ
QC Samples	10 ng/mL	20 ng/mL	160 ng/mL	1280 ng/mL
Accuracy	117 ± 3.8 %	109.7 ± 6.7 %	93.6 ± 0.4 %	99.3 ± 1.9 %
Precision	4.52 %CV	5.94 %CV	1.55 %CV	2.08 %CV
Linearity	10 – 1280 ng/mL
Recovery	104.2 ± 4.9 %	- - - - - - - - - - - - - - - - -	100.6 ± 0.9 %	102.9 ± 5.8 %
Stability	9.9 ± 0.6 ng/mL	20.3 ± 1.3 ng/mL	152.5 ± 8.7 ng/mL	1234 ± 33.0 ng/mL