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International Journal of Analytical Chemistry
Volume 2018, Article ID 3013497, 8 pages
Research Article

Optimum Extraction, Characterization, and Antioxidant Activities of Polysaccharides from Flowers of Dendrobium devonianum

1Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2Laboratory of Quality & Safety Risk Assessment on Agro-Products Processing (Beijing), Ministry of Agriculture, Beijing, China

Correspondence should be addressed to Fengzhong Wang; moc.anis@gnohzgnefgnaw

Received 29 September 2017; Accepted 1 January 2018; Published 8 February 2018

Academic Editor: Jiajia Rao

Copyright © 2018 Donghui Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Response surface methodology (RSM) was employed to optimize the conditions for the ultrasonic-assisted extraction (UAE) of polysaccharides from the flowers of Dendrobium devonianum. The optimal conditions for the maximum yields of DDFPs are as follows: an extraction temperature of 63.13°C, an extraction time of 53.10 min, and a water-to-raw material ratio of 22.11 mL/g. Furthermore, three fractions (DDFPs30, DDFPs50, and DDFPs70) were prepared from Dendrobium devonianum flowers polysaccharides (DDFPs) by the stepwise ethanol precipitation method. The DDFPs50 exhibited the highest antioxidant activity compared to the other fractions. The molecular weight, polydispersity, and conformation of these fractions were also characterized. In particular, the monosaccharide composition analysis of the DDFPs indicates that mannose and glucose are the primary components, similar to those of the D. officinale plant. This study provides a rapid extraction technology and essential information for the production of DDFPs, which could be potentially used as healthcare food.