Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis

Table 2

Retention factor () values of spots observed in the 1D-TLC plates, developed using the indicated mobile phases.

MP-1				MP-2					MP-3
	VL		UV		VL		UV		VL		UV
1	0.97	12	0.76	1	0.98	7	0.97	1	0.84	8	0.84
2	0.93	13	0.66	2	0.93	8	0.79	2	0.82	9	0.71
3	0.90	14	0.47	3	0.87	9	0.70	3	0.78	10	0.62
4	0.84	15	0.41	4	0.76	10	0.26	4	0.68	11	0.36
5	0.79	16	0.37	5	0.07	11	0.08	5	0.10	12	0.13
6	0.69	17	0.07	6	0.00	12	0.03	6	0.04	13	0.04
7	0.58					13	0	7	0.00	14	0
8	0.50
9	0.11
10	0.04
11	0.00
MP-4a				MP-4b					MP-4c
	VL		UV		VL		UV		VL		UV
1	0.92	7	0.92	1	0.83	8	0.83	1	0.89	8	0.81
2	0.84	8	0.79	2	0.77	9	0.71	2	0.87	9	0.59
3	0.51	9	0.23	3	0.69	10	0.53	3	0.77	10	0.39
4	0.43	10	0.19	4	0.32	11	0.33	4	0.70	11	0.18
5	0.07	11	0.04	5	0.11	12	0.22	5	0.49	12	0.00
6	0.00	12	0.00	6	0.06	13	0.07	6	0.04
				7	0.00	14	0	7	0
MP-5a				MP-5b				MP-5c
	VL		UV		VL		UV		VL		UV
1	0.94	16	0.92	1	0.96	16	0.93	1	0.96	16	0.93
2	0.92	17	0.44	2	0.93	17	0.70	2	0.93	17	0.70
3	0.88	18	0.37	3	0.91	18	0.46	3	0.91	18	0.59
4	0.80	19	0.29	4	0.58	19	0.38	4	0.77	19	0.49
5	0.41	20	0.26	5	0.54	20	0.28	5	0.74	20	0.43
6	0.36	21	0.16	6	0.50	21	0.16	6	0.68	21	0.39
7	0.32			7	0.42	22	0.09	7	0.52	22	0.32
8	0.24			8	0.32	23	0.00	8	0.50	23	0.27
9	0.20			9	0.26			9	0.48	24	0.17
10	0.13			10	0.21			10	0.42	25	0.04
11	0.11			11	0.18			11	0.38
12	0.10			12	0.14			12	0.30
13	0.09			13	0.07			13	0.28
14	0.04			14	0.03			14	0.11
15	0.00			15	0.00			15	0.00

Spots considered as well resolved.