Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis
Table 3
Retention factor () values observed in 2D-TLC plates, developed with the indicated mobile phases.
VL
UVL366
Spot
MP-1
MP-1
Spot
MP-1
MP-1
1
0.74
0.76
12
0.59
0.59
2
0.66
0.66
13
0.56
0.56
3
0.62
0.62
14
0.52
0.49
4
0.58
0.53
15
0.41
0.38
5
0.55
0.49
16
0.30
0.26
0.47
0.44
17
0.15
0.15
7
0.38
0.37
18
0.06
0.12
0.34
0.30
19
0.04
0.02
9
0.31
0.26
20
0.00
0.00
10
0.03
0.02
11
0.00
0.00
MP-5c
MP-4a
MP-5c
Mp-4a
1
0.79
0.79
19
0.46
0.59
2
0.74
0.86
20
0.46
0.09
3
0.68
0.59
21
0.38
0.06
4
0.64
0.69
22
0.34
0.35
5
0.64
0.54
23
0.26
0.35
6
0.60
0.71
24
0.26
0.03
7
0.60
0.53
25
0.16
0.02
8
0.56
0.88
26
0.11
0.00
9
0.56
0.68
27
0.05
0.00
10
0.50
0.43
28
0.00
0.00
11
0.45
0.48
2
0.20
0.26
1
0.45
0.29
13
0.38
0.43
1
0.38
0.18
15
0.30
0.09
1
0.22
0.00
17
0.15
0.00
18
0.04
0.00
Spots considered as well resolved. Spots considered as well-resolved and containing compounds with antioxidant activity.