Research Article

Two-Dimensional Thin Layer Chromatography-Bioautography Designed to Separate and Locate Metabolites with Antioxidant Activity Contained on Spirulina platensis

Table 3

Retention factor () values observed in 2D-TLC plates, developed with the indicated mobile phases.

VLUVL366
SpotMP-1MP-1SpotMP-1MP-1

10.740.76120.590.59
20.660.66130.560.56
30.620.62140.520.49
40.580.53150.410.38
50.550.49160.300.26
0.470.44170.150.15
70.380.37180.060.12
0.340.30190.040.02
90.310.26200.000.00
100.030.02
110.000.00

MP-5cMP-4aMP-5cMp-4a

10.790.79190.460.59
20.740.86200.460.09
30.680.59210.380.06
40.640.69220.340.35
50.640.54230.260.35
60.600.71240.260.03
70.600.53250.160.02
80.560.88260.110.00
90.560.68270.050.00
100.500.43280.000.00
110.450.4820.200.26
10.450.29
130.380.43
10.380.18
150.300.09
10.220.00
170.150.00
180.040.00

Spots considered as well resolved.
Spots considered as well-resolved and containing compounds with antioxidant activity.