Determination of Aflatoxin B₁ in Feedstuffs without Clean-Up Step by High-Performance Liquid Chromatography

Table 1

The results of validation method with mean value(accuracy and precision), n=6.

Matrices	Spiked level; (ng/g)	%Recovery			Intra-day (%)			Inter-day(%RS)	LOD; (ng/g)	LOQ; (ng/g)
		Day 1	Day 2	Day 3	Day 1	Day 2	Day 3
Broken rice	20	86.83	91.83	96.83	3.52	5.48	3.16	5.44	0.2	0.3
	40	82.50	89.83	90.83	3.73	4.30	4.25	4.12
	100	84.00	93.66	92.50	3.61	3.35	2.34	5.77
Peanut	20	106.57	109.37	109.37	3.34	5.89	1.98	1.42	0.6	0.9
	40	106.65	104.13	109.41	1.02	2.45	5.42	2.47
	100	101.64	93.89	107.10	4.06	1.83	3.97	6.58
Corn	20	90.10	90.98	91.72	6.77	5.63	4.89	0.89	0.6	0.8
	40	91.84	95.06	84.95	5.05	5.27	1.69	5.70
	100	86.36	85.64	85.43	3.28	4.20	6.33	0.57
Fish meal	20	109.85	108.88	102.31	5.02	2.96	6.18	6.33	1.2	1.5
	40	100.35	89.13	97.92	4.26	2.99	11.0	6.16
	100	86.63	86.70	99.49	2.76	2.34	2.38	8.29