Development of Isocratic RP-HPLC Method for Separation and Quantification of L-Citrulline and L-Arginine in Watermelons

<div>Comparative chromatograms showing isocratic RP-HPLC separation of mixed standard, L-citrulline, and L-arginine from 2 different columns: (a) Zorbax Eclipse XDB-C<sub>18</sub>, 5 <i>μ</i>m, and (b) Gemini C<sub>18</sub>, 3 <i>μ</i>m; efficient separation and the best resolution were achieved by the Gemini C<sub>18</sub> column which showed that compounds are well separated. The peaks marked represent (1) L-arginine and (2) L-citrulline.</div>

International Journal of Analytical Chemistry

fig4

Figure 4

Figure 4: Development of Isocratic RP-HPLC Method for Separation and Quantification of L-Citrulline and L-Arginine in Watermelons 